Overview

  • Product name

  • Description

    Rabbit polyclonal to STK3/MST-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human STK3/MST-2 (internal sequence). The exact sequence is proprietary.
    Database link: Q13188

  • Positive control

    • WB: THP-1, HEK-293T, A431 and NCI-H929 whole cell lysate. STK3/MST-2 transfected HEK-293T lysates.
  • General notes

    Previously labelled as STK3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab227152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 56 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.
  • Tissue specificity

    Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
  • Cellular localization

    Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0610042I06Rik antibody
    • EC 2.7.11.1 antibody
    • FLJ90748 antibody
    • KB 1458E12.1 antibody
    • Kinase responsive to stress 1 antibody
    • KRS1 antibody
    • Mammalian STE20 like protein kinase 2 antibody
    • Mammalian STE20-like protein kinase 2 antibody
    • Mammalian sterile 20-like 2 antibody
    • Mess1 antibody
    • MST antibody
    • MST-2 antibody
    • MST2 antibody
    • Mst3 antibody
    • Serine/threonine kinase 3 (STE20 homolog, yeast) antibody
    • Serine/threonine kinase 3 (Ste20, yeast homolog) antibody
    • Serine/threonine kinase 3 antibody
    • Serine/threonine protein kinase 3 antibody
    • Serine/threonine protein kinase Krs1 antibody
    • Serine/threonine-protein kinase 3 antibody
    • Serine/threonine-protein kinase Krs-1 antibody
    • STE20 like kinase MST2 antibody
    • STE20-like kinase MST2 antibody
    • Stk3 antibody
    • STK3_HUMAN antibody
    • wu:fc19e11 antibody
    • zgc:55383 antibody
    see all

Images

  • All lanes : Anti-STK3/MST-2 antibody (ab227152) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 56 kDa



    10% SDS-PAGE

  • STK3/MST-2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 4 µg of ab227152. Western blot was performed from the immunoprecipitate using ab227152 at a 1/500 diution.

    Lane 1: Control with 4 μg of preimmune Rabbit IgG.

    Lane 2: 4 µg ab227152 IP in HeLa whole cell extracts.

    7.5% SDS-PAGE.

  • All lanes : Anti-STK3/MST-2 antibody (ab227152) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysates
    Lane 2 : STK3/MST-2 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysates

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 56 kDa



    7.5% SDS PAGE

  • All lanes : Anti-STK3/MST-2 antibody (ab227152) at 1/1000 dilution

    Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 2 : NCI-H929 (human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 56 kDa



    7.5% SDS PAGE

References

ab227152 has not yet been referenced specifically in any publications.

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