Anti-STK3/MST-2 antibody (ab70546)
Key features and details
- Rabbit polyclonal to STK3/MST-2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-STK3/MST-2 antibody
See all STK3/MST-2 primary antibodies -
Description
Rabbit polyclonal to STK3/MST-2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human STK3/MST-2 (C terminal).
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Positive control
- Whole cell lysate from HeLa, Ramos or NIH3T3 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/5000 - 1/15000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/5000 - 1/15000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. -
Tissue specificity
Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain. -
Cellular localization
Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 533815 Cow
- Entrez Gene: 6788 Human
- Entrez Gene: 56274 Mouse
- Entrez Gene: 65189 Rat
- Entrez Gene: 432210 Xenopus laevis
- Entrez Gene: 100036637 Xenopus tropicalis
- Entrez Gene: 324125 Zebrafish
- Omim: 605030 Human
see all -
Alternative names
- 0610042I06Rik antibody
- EC 2.7.11.1 antibody
- FLJ90748 antibody
see all
Images
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All lanes : Anti-STK3/MST-2 antibody (ab70546) at 1/10000 dilution
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from Ramos cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling STK3/MST-2 with ab70546 at 1/1000 (1µg/ml). Detection: DAB.
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1mg whole cell lysate from HeLa cells was immunoprecipitated with ab70546 at 3ug/mg of lysate (lane 1) or a control rabbit Ig )lane 2). For the subsequent blot, 25% of the immnuoprecipitate (lanes 1 and 2) or the input (lane 3) were probed with ab70546 at 1mg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab70546 has been referenced in 2 publications.
- Brandt ZJ et al. Core Hippo pathway components act as a brake on Yap and Taz in the development and maintenance of the biliary network. Development 147:N/A (2020). PubMed: 32439761
- Chen FB et al. LINC01315 Impairs microRNA-211-Dependent DLG3 Downregulation to Inhibit the Development of Oral Squamous Cell Carcinoma. Front Oncol 10:556084 (2020). PubMed: 33117688