Overview

  • Product name

    Anti-STK40 antibody - C-terminal
  • Description

    Rabbit polyclonal to STK40 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, selected from the C-terminal region of Human STK40.

  • Positive control

    • 293 cell lysates transiently transfected with the STK40 gene; Mouse brain cerebellum tissue; 293 cells. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: A431

Properties

Applications

Our Abpromise guarantee covers the use of ab135747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Predicted molecular weight: 49 kDa.

Target

  • Relevance

    STK40 may be a negative regulator of NF-kappa-B and p53-mediated gene transcription. There are four named isoforms.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • 2310004N11Rik antibody
    • MGC4796 antibody
    • OTTHUMP00000009948 antibody
    • OTTHUMP00000009951 antibody
    • RP11-268J15.4 antibody
    • Ser/Thr like kinase antibody
    • Serine/threonine kinase 40 antibody
    • Serine/threonine protein kinase 40 antibody
    • SgK495 antibody
    • SHIK antibody
    • SINK homologous serine/threonine protein kinase antibody
    • SINK-homologous inhibitory kinase antibody
    • STK 40 antibody
    • Sugen kinase 495 antibody
    see all

Images

  • ICC/IF image of ab135747 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135747 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-STK40 antibody - C-terminal (ab135747) at 1/50 dilution

    Lane 1 : 293 cell lysates, non-transfected
    Lane 2 : 293 cell lysates, transiently transfected with the STK40 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 49 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse brain cerebellum tissue labelling STK40 with ab135747 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
  • Flow Cytometric analysis of 293 cells labelling STK40 with ab135747 at 1/10 dilution (green) compared to a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab135747 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab135747.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up