Overview

  • Product name
    Anti-STOM antibody - N-terminal
    See all STOM primary antibodies
  • Description
    Goat polyclonal to STOM - N-terminal
  • Host species
    Goat
  • Specificity
    ab169834 is expected to recognize both reported isoforms (NP_004090.4; NP_937837.1).
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    EKRHTRDSEAQR

    by a Cysteine residue linker, corresponding to N terminal amino acids 3-14 of Human STOM (NP_004090.4; NP_937837.1.)

  • Positive control
    • Human erythroocytes, spleen, bone marrow and lung lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99.48% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab169834 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).

Target

  • Function
    Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the band 7/mec-2 family.
  • Cellular localization
    Cell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • BND7 antibody
    • EPB7 antibody
    • EPB72 antibody
    • Erythrocyte band 7 integral membrane protein antibody
    • Erythrocyte membrane protein band 7.2 (stomatin) antibody
    • Erythrocyte surface protein band 7.2 antibody
    • Protein 7.2b antibody
    • STOM antibody
    • STOM_HUMAN antibody
    • Stomatin antibody
    see all

Images

  • ab169834 (5µg/ml) staining of paraffin embedded Human Liver following steamed antigen retrieval with citrate buffer pH 6 and AP-staining showing membrane staining of hepatocytes and of sinusoidal lining cells.

  • Anti-STOM antibody - N-terminal (ab169834) at 0.1 µg/ml + Human lung lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 32 kDa

References

ab169834 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab169834.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up