The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
Belongs to the band 7/mec-2 family.
Cell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ab169834 (5µg/ml) staining of paraffin embedded Human Liver following steamed antigen retrieval with citrate buffer pH 6 and AP-staining showing membrane staining of hepatocytes and of sinusoidal lining cells.
Western blot - Anti-Stomatin antibody (ab169834)
Anti-Stomatin antibody (ab169834) at 0.1 µg/ml + Human lung lysate in RIPA buffer at 35 µg