Recombinant
RabMAb

Anti-Stomatin antibody [EPR10420] (ab169524)

Overview

  • Product name
    Anti-Stomatin antibody [EPR10420]
    See all Stomatin primary antibodies
  • Description
    Rabbit monoclonal [EPR10420] to Stomatin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Stomatin.
    Database link: P27105

  • Positive control
    • Human fetal heart, HeLa and HepG2 lysates; HeLa cells; Immunoprecipitation pellet from HepG2 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as STOM. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10420
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the band 7/mec-2 family.
    • Cellular localization
      Cell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • BND7 antibody
      • EPB7 antibody
      • EPB72 antibody
      • Erythrocyte band 7 integral membrane protein antibody
      • Erythrocyte membrane protein band 7.2 (stomatin) antibody
      • Erythrocyte surface protein band 7.2 antibody
      • Protein 7.2b antibody
      • STOM antibody
      • STOM_HUMAN antibody
      • Stomatin antibody
      see all

    Images

    • All lanes : Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : HeLa lysate
      Lane 3 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 32 kDa

    • Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

      Predicted band size: 32 kDa

    • Immunofluorescent analysis of HeLa cells labeling Stomatin with ab169524 at 1/100 dilution. DAPI nuclear staining (blue).

    • Flow cytometric analysis of permeabilized HeLa cells labeling Stomatin with ab169524 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab169524 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169524.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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