Product nameAnti-Stomatin antibody [EPR10420]
See all Stomatin primary antibodies
DescriptionRabbit monoclonal [EPR10420] to Stomatin
Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Stomatin.
Database link: P27105
- Human fetal heart, HeLa and HepG2 lysates; HeLa cells; Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900).
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as STOM.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 32 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the band 7/mec-2 family.
Cellular localizationCell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- BND7 antibody
- EPB7 antibody
- EPB72 antibody
All lanes : Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 32 kDa
Immunofluorescent analysis of HeLa cells labeling Stomatin with ab169524 at 1/100 dilution. DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized HeLa cells labeling Stomatin with ab169524 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
ab169524 has not yet been referenced specifically in any publications.