Recombinant Anti-Stomatin antibody [EPR10420] (ab169524)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10420] to Stomatin
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Stomatin antibody [EPR10420]
See all Stomatin primary antibodies -
Description
Rabbit monoclonal [EPR10420] to Stomatin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Stomatin.
Database link: P27105 -
Positive control
- Human fetal heart, HeLa and HepG2 lysates; HeLa cells; Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10420 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the band 7/mec-2 family. -
Cellular localization
Cell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 2040 Human
- Omim: 133090 Human
- SwissProt: P27105 Human
- Unigene: 253903 Human
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Alternative names
- BND7 antibody
- EPB7 antibody
- EPB72 antibody
see all
Images
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All lanes : Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa -
Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 32 kDa -
Immunofluorescent analysis of HeLa cells labeling Stomatin with ab169524 at 1/100 dilution. DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Stomatin with ab169524 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab169524 has been referenced in 2 publications.
- Duan J et al. Targeted core-shell nanoparticles for precise CTCF gene insert in treatment of metastatic breast cancer. Bioact Mater 11:1-14 (2022). PubMed: 34938908
- An H et al. Stomatin plays a suppressor role in non-small cell lung cancer metastasis. Chin J Cancer Res 31:930-944 (2019). PubMed: 31949395