Recombinant
RabMAb

Anti-Stomatin antibody [EPR10421] (ab166623)

Overview

  • Product name
    Anti-Stomatin antibody [EPR10421]
    See all Stomatin primary antibodies
  • Description
    Rabbit monoclonal [EPR10421] to Stomatin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Stomatin.

  • Positive control
    • Human fetal heart, HeLa, HepG2, Neuro-2a and Raw264.7 lysates, HeLa cells, permeabilized Neuro-2a cells, immunoprecipitation pellet from fetal heart lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10421
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the band 7/mec-2 family.
    • Cellular localization
      Cell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • BND7 antibody
      • EPB7 antibody
      • EPB72 antibody
      • Erythrocyte band 7 integral membrane protein antibody
      • Erythrocyte membrane protein band 7.2 (stomatin) antibody
      • Erythrocyte surface protein band 7.2 antibody
      • Protein 7.2b antibody
      • STOM antibody
      • STOM_HUMAN antibody
      • Stomatin antibody
      see all

    Images

    • All lanes : Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution

      Lane 1 : Human fetal heart lysates
      Lane 2 : HeLa lysates
      Lane 3 : HepG2 lysates
      Lane 4 : Neuro-2a lysates
      Lane 5 : Raw264.7 lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 32 kDa

    • Immunofluorescence analysis of HeLa cells labeling STOM with ab166623 at 1/100 dilution.
    • Flow cytometric analysis of permeabilized Neuro-2a cells using ab166623 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
    • Immunoprecipitation of STOM in pellet from Human fetal heart lysate using ab166623 at 1/10 dilution, followed by WB analysis of STOM.

      Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution + immunoprecipitation pellet from Human fetal heart lysate at 10 µg

    References

    ab166623 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab166623.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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