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Rabbit monoclonal [EPR10421] to Stomatin
Flow Cyt more details Unsuitable for:
Mouse, Rat, Human
corresponding to Human Stomatin.
Human fetal heart, HeLa, HepG2, Neuro-2a and Raw264.7 lysates, HeLa cells, permeabilized Neuro-2a cells, immunoprecipitation pellet from fetal heart lysate
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
1/100 - 1/250.
1/10 - 1/100.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IHC-P.
Thought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
Belongs to the band 7/mec-2 family.
Cell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Information by UniProt
Western blot - Anti-Stomatin antibody [EPR10421] (ab166623)
All lanes : Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution Lane 1 : Human fetal heart lysates Lane 2 : HeLa lysates Lane 3 : HepG2 lysates Lane 4 : Neuro-2a lysates Lane 5 : Raw264.7 lysates Lysates/proteins at 10 µg per lane. Predicted band size: 32 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10421] (ab166623)
Immunofluorescence analysis of HeLa cells labeling STOM with ab166623 at 1/100 dilution.
Flow Cytometry - Anti-Stomatin antibody [EPR10421] (ab166623)
Flow cytometric analysis of permeabilized Neuro-2a cells using ab166623 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
Immunoprecipitation - Anti-Stomatin antibody [EPR10421] (ab166623)
Immunoprecipitation of STOM in pellet from Human fetal heart lysate using ab166623 at 1/10 dilution, followed by WB analysis of STOM.
Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution + immunoprecipitation pellet from Human fetal heart lysate at 10 µg
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"