Product nameAnti-Stomatin antibody [EPR10421]
See all Stomatin primary antibodies
DescriptionRabbit monoclonal [EPR10421] to Stomatin
Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Stomatin.
Database link: P27105
- Human fetal heart, HeLa, HepG2, Neuro-2a and Raw264.7 lysates, HeLa cells, permeabilized Neuro-2a cells, immunoprecipitation pellet from fetal heart lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab166623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 32 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThought to regulate cation conductance. May regulate ACCN1 and ACCN3 gating.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the band 7/mec-2 family.
Cellular localizationCell membrane. Cell membrane. Melanosome. Exposed on the cytoplasmic surface of the membrane. Associated with lipid rafts. Concentrates preferentially in plasma membrane protrusions and in a juxta-nuclear region which may represent Golgi-derived vesicles. Colocalizes with actin. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- BND7 antibody
- EPB7 antibody
- EPB72 antibody
All lanes : Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution
Lane 1 : Human fetal heart lysates
Lane 2 : HeLa lysates
Lane 3 : HepG2 lysates
Lane 4 : Neuro-2a lysates
Lane 5 : Raw264.7 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa
Immunofluorescence analysis of HeLa cells labeling Stomatin with ab166623 at 1/100 dilution.
Flow cytometric analysis of permeabilized Neuro-2a cells using ab166623 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
Immunoprecipitation of Stomatin in pellet from Human fetal heart lysate using ab166623 at 1/10 dilution, followed by WB analysis of Stomatin.
Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution + immunoprecipitation pellet from Human fetal heart lysate at 10 µg
ab166623 has not yet been referenced specifically in any publications.