Overview

  • Product name

    Anti-STON2 antibody
  • Description

    Rabbit polyclonal to STON2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 299-437 of Human STON2.

  • Positive control

    • Human fallopian tube tissue; A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 fixation.
WB 1/250 - 1/500. Predicted molecular weight: 101 kDa.

Target

  • Function

    Adapter protein involved in endocytic machinery. May be involved in the vesicle recycling. May facilitate clathrin-coated vesicle uncoating.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the Stoned B family.
    Contains 1 MHD (mu homology) domain.
    Contains 1 SHD (stonin homology) domain.
  • Domain

    The Asn-Pro-Phe (NPF) motifs, which are found in proteins involved in the endocytic pathway, mediate the interaction with the EH domain of SYT1, SYT2, EPS15, EPS15R and ITSN1.
  • Cellular localization

    Cytoplasm. Membrane. Some fraction is membrane-associated.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4933401N24Rik antibody
    • AI425999 antibody
    • AW544599 antibody
    • Homolog of stoned B antibody
    • RGD1311502 antibody
    • STN2 antibody
    • STNB antibody
    • STNB2 antibody
    • STON2 antibody
    • STON2_HUMAN antibody
    • Stoned B antibody
    • Stonin-2 antibody
    see all

Images

  • Anti-STON2 antibody (ab121165) + Human cell line K562

    Predicted band size: 101 kDa

  • ab121165 at 1/125 dilution staining STON2 in Paraffin-emebedded Human fallopian tube tissue by Immunohistochemistry.

    Note: moderate cytoplasmic positivity in glandular cells.
  • ab121165 at 4 µg/ml staining STON2 in PFA/Triton X-100 fixed A431 cells by Immunofluorescence(green).

    Note: positivity in nucleoli and cytoplasm.

References

ab121165 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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