Anti-STOX1 antibody - C-terminal (ab189436)
Key features and details
- Rabbit polyclonal to STOX1 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STOX1 antibody - C-terminal
See all STOX1 primary antibodies -
Description
Rabbit polyclonal to STOX1 - C-terminal -
Host species
Rabbit -
Specificity
At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. ab189436 will recognize the longest isoform. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human STOX1 (C terminal). Raised against a 14 amino acid peptide near the C-terminal end.
Database link: Q6ZVD7 -
Positive control
- Human liver lysate. Human adrenal or skeletal muscle tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab189436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 111 kDa. |
Target
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Involvement in disease
Defects in STOX1 are the cause of pre-eclampsia/eclampsia 4 (PEE4) [MIM:609404]; also known as gestational proteinuric hypertension. Preeclampsia is a pregnancy-associated disease with maternal symptoms but placental origin. Unlike most other human disorders, it impacts 2 individuals, the mother and her child, both of whom can be severely affected. The pathological mechanism of PEE4 involves the loss of function of STOX1 in the placenta. -
Cellular localization
Nucleus; Nucleus > nucleolus and Cytoplasm. Nucleus. Either found in the cytoplasm or in the nucleus. Nuclear expression of isoform A seems to be restricted to polyploid trophoblast cells. - Information by UniProt
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Database links
- Entrez Gene: 219736 Human
- Omim: 609397 Human
- SwissProt: Q6ZVD7 Human
- Unigene: 37636 Human
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Alternative names
- C10orf24 antibody
- chromosome 10 open reading frame 241 antibody
- PEE4 antibody
see all
Images
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Western blot - Anti-STOX1 antibody - C-terminal (ab189436)All lanes : Anti-STOX1 antibody - C-terminal (ab189436) at 1 µg/ml
Lane 1 : Human liver lysate
Lane 2 : Human liver lysate plus blocking peptide
Predicted band size: 111 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STOX1 antibody - C-terminal (ab189436)Immunohistochemical analysis of formalin fixed, paraffin embedded human skeletal muscle labeling STOX1 with ab189436 at 5 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STOX1 antibody - C-terminal (ab189436)Immunohistochemical analysis of formalin fixed, paraffin embedded human adrenal tissue labeling STOX1 with ab189436 at 5 μg/ml.
Protocols
Datasheets and documents
References (0)
ab189436 has not yet been referenced specifically in any publications.
