Key features and details
- Goat polyclonal to Stra6
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 74 kDa).
A 1 hour primary incubation is recommended for this product. Please note a non-specific band of ~80kDa.
FunctionMay act as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). Acts by removing retinol from RBP/RBP4 and transports it across the plasma membrane, where it can be metabolized. This mechanism does not depend on endocytosis. Binds to RBP/RBP4 with high affinity. Increases cellular retinol uptake from the retinol-RBP complex.
Tissue specificityBroad expression. In adult eye expressed in sclera, retina, retinal pigment epithelium, and trabecular meshwork but not in choroid and iris.
Involvement in diseaseDefects in STRA6 are the cause of microphthalmia syndromic type 9 (MCOPS9) [MIM:601186]; also called Matthew-Wood syndrome or Spear syndrome. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS9 is a rare clinical entity including as main characteristics anophthalmia or severe microphthalmia, and pulmonary hypoplasia or aplasia.
Note=Mutations in STRA6 may be a cause of isolated colobomatous microphthalmia, a disorder of the eye characterized by an abnormally small ocular globe.
Cellular localizationCell membrane.
- Information by UniProt
- MCOPCB8 antibody
- MCOPS9 antibody
- PP14296 antibody
All lanes : Anti-Stra6 antibody (ab77616) at 1 µg/ml
Lane 1 : Rat testes tissue lysate
Lane 2 : Rat testes tissue lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 74 kDa
Observed band size: 80-90 kDa why is the actual band size different from the predicted?
Primary incubation 1 hour at room temperature. Detected by chemiluminescence.
ab77616 has not yet been referenced specifically in any publications.