Key features and details
- Rabbit polyclonal to STRAD
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-STRAD antibody
See all STRAD primary antibodies
DescriptionRabbit polyclonal to STRAD
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Cow, Macaque monkey, Orangutan
Recombinant fragment corresponding to Human STRAD aa 25-210.
RDLELFGEQPPGDTRRKTNDASSESIASFSKQEVMSSFLPEGGCYELLTV IGKGFEDLMTVNLARYKPTGEYVTVRRINLEACSNEMVTFLQGELHVSKL FNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLICTHFMDGMNELAIAY ILQGVLKALDYIHHMGYVHRSVKASHILISVDGKVY
Database link: Q7RTN6
- WB: Rat heart tissue lysate. IHC-P: Human brain and kidney tissue.
This product was previously labelled as LYK5
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 48 kDa.|
RelevanceMembers of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1.
Cellular localizationCytoplasmic and Nuclear
- Protein kinase LYK5 antibody
- Serologically defined breast cancer antigen NY BR 96 antibody
- STE20 related adapter protein antibody
Anti-STRAD antibody (ab230118) at 1/1000 dilution + Rat heart tissue lysate
Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Paraffin-embedded human brain tissue stained for STRAD using ab230118 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for STRAD using ab230118 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230118 has not yet been referenced specifically in any publications.