Overview

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity chromatography purified via peptide column
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 24 kDa.

Target

  • Relevance

    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
  • Alternative names

    • anti-Strep-Tag II antibody
    • Strep-Tag II antibody
    • Strep-Tag II antibody antibody
    • Strep-Tag IIantiboy antibody

Images

  • All lanes : Anti-Strep-tag II antibody (ab76950) at 1 µg/ml

    Lane 1 : Streptavidin tagged recombinant protein with blocking peptide
    Lane 2 : Streptavidin tagged recombinant protein

    Lysates/proteins at 0.05 µg per lane.

    Predicted band size: 24 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Baggett NE  et al. Global analysis of translation termination in E. coli. PLoS Genet 13:e1006676 (2017). Read more (PubMed: 28301469) »
  • Lin Y  et al. Antiaging Gene Klotho Deficiency Promoted High-Fat Diet-Induced Arterial Stiffening via Inactivation of AMP-Activated Protein Kinase. Hypertension 67:564-73 (2016). Read more (PubMed: 26781278) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

We have two different versions of the tag: ab76950- against GGGWSHPQFEK ab76949- against ASWSHPQFEKGA Both contain the tag sequence WSHPQFEK along with extra inert amino acids to make the sequence longer for conjugation chemistry to the carrier protein and purification column. They should not interfere with antibody specificity, but if the customer is concerned, the immunogen for ab76950 would highlight the tag the most.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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