Recombinant
RabMAb

Recombinant Anti-Strep-tag II antibody [EPR12666] - BSA and Azide free (ab232586)

Overview

  • Product name

    Anti-Strep-tag II antibody [EPR12666] - BSA and Azide free
    See all Strep-tag II primary antibodies
  • Description

    Rabbit monoclonal [EPR12666] to Strep-tag II - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to Strep-tag II.

  • Positive control

    • WB: Strep-tag II protein.
  • General notes

    Ab232586 is the carrier-free version of ab180957. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232586 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12666
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab232586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 16-100 kDa.

Target

  • Relevance

    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
  • Alternative names

    • anti-Strep-Tag II antibody
    • Strep-Tag II antibody
    • Strep-Tag II antibody antibody
    • Strep-Tag IIantiboy antibody

Images

  • All lanes : Anti-Strep-tag II antibody [EPR12666] (ab180957) at 1/1000 dilution (Unpurified)

    Lane 1 : Strep-tag II protein at 0.01 µg
    Lanes 2 & 4 : Strep-tag II protein at 0.005 µg
    Lane 3 : Strep-tag II protein at 0.001 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180957).

References

ab232586 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. I am sorry but without understanding the precise epigenetic profiling and present literature it is difficult for me to suggest antibodies for ChIP analysis of this region. Standardly I can recommend that your customer analyses the levels of histone H3 tri-methylated K4 and di-methylated-K9. However, they may wish to examine for the presence of TBP and various transcription machiney in addition to specific transcription factors. However, not knowing the background to the gene it is difficult for me to comment.

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