Product nameAnti-Strep-tag II antibody [GT661]
See all Strep-tag II primary antibodies
DescriptionMouse monoclonal [GT661] to Strep-tag II
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Species independent
- Strep-tagged protein expressing 293T cells.
This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab184224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000.|
|ICC/IF||1/100 - 1/1000.|
RelevanceStreptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
- anti-Strep-Tag II antibody
- Strep-Tag II antibody
- Strep-Tag II antibody antibody
- Strep-Tag IIantiboy antibody
All lanes : Anti-Strep-tag II antibody [GT661] (ab184224) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : whole cell lysate of strep-tagged II protein expressing 293T cells
Lysates/proteins at 30 µg per lane.
12 % SDS-PAGE
Immunofluorescent analysis of Strep II/GFP-tag transfected HeLa cells (fixed in 4% paraformaldehyde at RT for 15 min) showing labeling of Strep-recombinant protein by ab184224 used at 1/500 dilution. Red: Strep-recombinant protein stained by Strep-tag antibody (ab184224) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.
ab184224 immunoprecipitates strep tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract.
Lane 1 : 20 μg whole cell lysate/extract of strep II tagged protein expressing 293T cells
Lane 2 : Control with 2.5 μg of pre-immune mouse IgG
Lane 3 : Immunoprecipitation of strep tagged protein by 2.5 μg of strep tag antibody (ab184224).
10% SDS-PAGE -The immunoprecipitated strep tagged protein was detected by strep tag antibody (ab184224) diluted at 1/3000. Anti-mouse IgG (HRP) antibody was used as a secondary reagent.
This product has been referenced in:
- Lulla V et al. An upstream protein-coding region in enteroviruses modulates virus infection in gut epithelial cells. Nat Microbiol 4:280-292 (2019). Read more (PubMed: 30478287) »
- Brenca M et al. NR4A3 fusion proteins trigger an axon guidance switch that marks the difference between EWSR1 and TAF15 translocated extraskeletal myxoid chondrosarcomas. J Pathol N/A:N/A (2019). Read more (PubMed: 31020999) »