Anti-Strep-tag II antibody [GT661] (ab184224)

Submit a review Q&A (1)References (6)
Western blot - Anti-Strep-tag II antibody [GT661] (ab184224)
  • Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [GT661] (ab184224)
  • Immunoprecipitation - Anti-Strep-tag II antibody [GT661] (ab184224)

Key features and details

  • Mouse monoclonal [GT661] to Strep-tag II
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Strep-tag II antibody [GT661]
    See all Strep-tag II primary antibodies

  • Description

    Mouse monoclonal [GT661] to Strep-tag II

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to Strep-tag II.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Strep-tagged protein expressing 293T cells.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    GT661

  • Isotype

    IgG2a

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000.
IP
1/3000.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/1000 - 1/10000.
IP
1/3000.
ICC/IF
1/100 - 1/1000.

Target

  • Relevance

    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.

  • Alternative names

    • anti-Strep-Tag II antibody
    • Strep-Tag II antibody
    • Strep-Tag II antibody antibody
    • Strep-Tag IIantiboy antibody

Images

  • Western blot - Anti-Strep-tag II antibody [GT661] (ab184224)
    Western blot - Anti-Strep-tag II antibody [GT661] (ab184224)
    All lanes : Anti-Strep-tag II antibody [GT661] (ab184224) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : whole cell lysate of strep-tagged II protein expressing 293T cells

    Lysates/proteins at 30 µg per lane.


    12 % SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [GT661] (ab184224)
    Immunocytochemistry/ Immunofluorescence - Anti-Strep-tag II antibody [GT661] (ab184224)

    Immunofluorescent analysis of Strep II/GFP-tag transfected HeLa cells (fixed in 4% paraformaldehyde at RT for 15 min) showing labeling of Strep-recombinant protein by ab184224 used at 1/500 dilution. Red: Strep-recombinant protein stained by Strep-tag antibody (ab184224) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.

  • Immunoprecipitation - Anti-Strep-tag II antibody [GT661] (ab184224)
    Immunoprecipitation - Anti-Strep-tag II antibody [GT661] (ab184224)

    ab184224 immunoprecipitates strep tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract.

    Lane 1 : 20 μg whole cell lysate/extract of strep II tagged protein expressing 293T cells

    Lane 2 : Control with 2.5 μg of pre-immune mouse IgG

    Lane 3 : Immunoprecipitation of strep tagged protein by 2.5 μg of strep tag antibody (ab184224).

    10% SDS-PAGE -The immunoprecipitated strep tagged protein was detected by strep tag antibody (ab184224) diluted at 1/3000. Anti-mouse IgG (HRP) antibody was used as a secondary reagent.

References (6)

Publishing research using ab184224? Please let us know so that we can cite the reference in this datasheet.

ab184224 has been referenced in 6 publications.

  • Lulla V  et al. An upstream protein-coding region in enteroviruses modulates virus infection in gut epithelial cells. Nat Microbiol 4:280-292 (2019). PubMed: 30478287
  • Brenca M  et al. NR4A3 fusion proteins trigger an axon guidance switch that marks the difference between EWSR1 and TAF15 translocated extraskeletal myxoid chondrosarcomas. J Pathol N/A:N/A (2019). PubMed: 31020999
  • Habich M  et al. Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay. Cell Rep 26:759-774.e5 (2019). PubMed: 30650365
  • Li CG  et al. PPAR? Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis. Cell Rep 26:1333-1343.e7 (2019). PubMed: 30699358
  • Li M  et al. Identification of antiviral roles for the exon-junction complex and nonsense-mediated decay in flaviviral infection. Nat Microbiol 4:985-995 (2019). PubMed: 30833725
  • Fontaine KA  et al. The Cellular NMD Pathway Restricts Zika Virus Infection and Is Targeted by the Viral Capsid Protein. MBio 9:N/A (2018). PubMed: 30401782

Customer reviews and Q&As

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Submit a question

Question

Is the antibody Anti-Strep Tag cross-reacts with Strep II tag as well?

Read More
Answer

The immunogen of ab184224 was taken from Strep II proteins therefore, this antibody should bind to Srept II protein as well.

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