Product nameStreptavidin Antibody Conjugation Check Kit
Streptavidin Antibody Conjugation Check Kit (ab270051) is a quick and simple immunochromatography test that allows you to confirm the successful conjugation of streptavidin to your antibody without the need for any specialized or costly equipment.
This product is manufactured by Expedeon, an Abcam company. It was previously called Streptavidin Check&Go! Antibody Labeling Confirmation Kit. Expedeon product code 4004-0030 is the same as the 30 test size of this product.
The key component of the kit is a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip”. Both Protein A and Protein G have a high affinity for the Fc region of a variety of IgG molecules (see Appendix). The “half strips” also contain an absorbent pad to promote and control the flow of sample through the nitrocellulose. The streptavidin-antibody conjugate is run on the Protein A/G strip together with 40nm gold conjugated Biotin as detection label.
Biotin binds streptavidin with exceptionally high affinity and when the streptavidin-antibody conjugate flows along the nitrocellulose membrane, upon binding to the Protein A and Protein G on the T-line, a visible red line appears on the strip.
Storage instructionsPlease refer to protocols.
Components 30 tests 10X Running Buffer 2 vials 40nm Gold Conjugated Biotin (lyophilized) 1 vial Protein A/G Strips 30 tests Streptavidin-Rabbit IgG Conjugate (lyophilized) 1 vial
ab270051 has not yet been referenced specifically in any publications.