Product nameAnti-Streptavidin antibody [S10D4]
See all Streptavidin primary antibodies
DescriptionMouse monoclonal [S10D4] to Streptavidin
SpecificityDoes not cross react with egg white avidin. Binds to Streptavidin without interfering with the biotin-binding reaction.
Tested applicationsSuitable for: ICC, WB, IHC-P, IHC-Fr, ICC/IF, Other, ELISA, Flow Cytmore details
Species reactivityReacts with: Species independent
Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product by June 2020 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We suggest ab191338 as a possible replacement.
Concentration varies from lot to lot and can be provided on request.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
Purification notesPurified from ascites.
Our Abpromise guarantee covers the use of ab10020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Other||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|AP||Use at an assay dependent concentration.|
RelevanceStreptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
- SA V1 antibody
- SA V2 antibody
- strepavidin antibody
Immunofluorescence analysis of Potorous tridactylis kidney (PtK2) cells, staining Streptavidin with ab10020.
Cells were fixed with 4% paraformaldehyde, before being incubated with wt streptavidin (2 µM) for 5 min at room temperature. Samples were incubated with 1 µL primary antibody for 1 hour at 4°C. A FITC-conjugated goat anti-mouse IgG was used as the secondary antibody.
This product has been referenced in:
- Gao S et al. Reciprocal Regulation Between O-GlcNAcylation and ß-Catenin Facilitates Cell Viability and Inhibits Apoptosis in Liver Cancer. DNA Cell Biol 38:286-296 (2019). Read more (PubMed: 30762425) »
- Bertuccio CA et al. Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent. PLoS One 9:e92013 (2014). Read more (PubMed: 24632741) »