Product nameAnti-Streptavidin antibody [S10D4]
See all Streptavidin primary antibodies
DescriptionMouse monoclonal [S10D4] to Streptavidin
SpecificityDoes not cross react with egg white avidin. Binds to Streptavidin without interfering with the biotin-binding reaction.
Tested applicationsSuitable for: ICC, WB, IHC-P, IHC-Fr, ICC/IF, Other, ELISA, Flow Cytmore details
Concentration varies from lot to lot and can be provided on request.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
PurityProtein A purified
Purification notesPurity tested by elcetrophoresis.
Our Abpromise guarantee covers the use of ab10020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Other||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|AP||Use at an assay dependent concentration.|
RelevanceStreptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
- SA V1 antibody
- SA V2 antibody
- strepavidin antibody
Immunofluorescence analysis of Potorous tridactylis kidney (PtK2) cells, staining Streptavidin with ab10020.
Cells were fixed with 4% paraformaldehyde, before being incubated with wt streptavidin (2 µM) for 5 min at room temperature. Samples were incubated with 1 µL primary antibody for 1 hour at 4°C. A FITC-conjugated goat anti-mouse IgG was used as the secondary antibody.
This product has been referenced in:
- Bertuccio CA et al. Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent. PLoS One 9:e92013 (2014). Read more (PubMed: 24632741) »
- Dykstra T et al. Defined particle ligands trigger specific defense mechanisms of macrophages. Innate Immun 17:388-402 (2011). Read more (PubMed: 20682584) »