Overview

  • Product name
    Anti-Streptavidin antibody [S10D4]
    See all Streptavidin primary antibodies
  • Description
    Mouse monoclonal [S10D4] to Streptavidin
  • Host species
    Mouse
  • Specificity
    Does not cross react with egg white avidin. Binds to Streptavidin without interfering with the biotin-binding reaction.
  • Tested applications
    Suitable for: ICC, WB, IHC-P, IHC-Fr, ICC/IF, Other, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Streptavidin.

  • General notes

    Concentration varies from lot to lot and can be provided on request.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Ascites
  • Purification notes
    Purified from ascites.
  • Clonality
    Monoclonal
  • Clone number
    S10D4
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

AP Use at an assay dependent concentration.

Target

  • Relevance
    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • SA V1 antibody
    • SA V2 antibody
    • strepavidin antibody
    • Streptavidin V1 antibody
    • Streptavidin V2 antibody
    see all

Images

  • Immunofluorescence analysis of Potorous tridactylis kidney (PtK2) cells, staining Streptavidin with ab10020.

    Cells were fixed with 4% paraformaldehyde, before being incubated with wt streptavidin (2 µM) for 5 min at room temperature. Samples were incubated with 1 µL primary antibody for 1 hour at 4°C. A FITC-conjugated goat anti-mouse IgG was used as the secondary antibody.

References

This product has been referenced in:
  • Bertuccio CA  et al. Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent. PLoS One 9:e92013 (2014). Read more (PubMed: 24632741) »
  • Dykstra T  et al. Defined particle ligands trigger specific defense mechanisms of macrophages. Innate Immun 17:388-402 (2011). Read more (PubMed: 20682584) »
See all 4 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8.5% gel)
Sample
Human Cell lysate - whole cell (HEK 293)
Specification
HEK 293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 18 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (hippocampal neurons)
Specification
hippocampal neurons
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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