Product nameStreptavidin Atto 542 (Monovalent)
Streptavidin Atto 542 (Monovalent)(ab272188) provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. The antibody- Streptavidin Atto 542 (Monovalent) complex is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.
Streptavidin Atto 542 (Monovalent) is a unique immunolabeling tool as it enables multiplexing with multiple biotinylated antibodies. There is no spillover of Streptavidin Atto 542 (Monovalent) reagent between antibodies and antibodies of the same animal host species can be used.
The Streptavidin Atto 542 (Monovalent) labeling reaction is efficient and can be run in such small volumes that it becomes practical to label individual antibodies with fluorochromes depending on the experiment.
Kits are available with 4 different fluorescent labels:
Streptavidin Alexa Fluor® 488 (Monovalent) (ab272187)
Streptavidin Atto 542 (Monovalent) (ab272188) - this kit
Streptavidin Alexa Fluor® 594 (Monovalent) (ab272189)
Streptavidin Alexa Fluor® 647 (Monovalent) (ab272190)
Tested applicationsSuitable for: Flow Cyt, IHCmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 50 µg 25 µg Biotin block buffer 1 x 500µl 1 x 250µl Streptavidin Atto 542 labeling reagent (Monovalent) 1 x 500µl 1 x 250µl
Our Abpromise guarantee covers the use of ab272188 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration.|
|IHC||Use at an assay dependent concentration.|
Multiplex immunostaining of a frozen tissue section of mouse spleen using four biotinylated antibodies labeled using Streptavidin (Monovalent) kits. T cells (rat anti-CD3, 7 µg/ml, green), B cells (rat anti-CD45R, 7 µg/ml, red), macrophages (rat anti-CD11b, 7 µg/ml, white), and dendritic cells (hamster anti-CD11c, 7 µg/ml, blue) were detected using Streptavidin (Monovalent) kits with four different fluorochromes. Picture was taken with a standard fluorescence microscope equipped with appropriate filters for optimal multicolor separation.
Antibodies labeled with Streptavidin (Monovalent) kits are as bright as directly conjugated antibodies. Flow cytometry analysis of splenic B lymphocytes immunolabeled with antibodies against CD45R (B220, clone RA3-6B2, 7 µg/ml). Dark green = directly conjugated antibody, light green = Streptavidin (Monovalent)-labeled antibody.
ab272188 has not yet been referenced specifically in any publications.