• Product name
    Streptavidin Conjugation Kit
  • Product overview

    Abcam's Streptavidin Conjugation Kit provides a simple and quick process to conjugate your primary antibodies with Streptavidin.

    The conjugated antibody can be used straight away in WB, ELISA, IHC etc

    Learn more about buffer compatibility, protein/secondary antibody conjugation and labelling chemistry in our FAQs.


  • Notes

    The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.


    Pre-conugation considerations

    The purified antibody to be labeled should ideally be in 10-50mM amine-free buffer (e.g. MES, MOPS, HEPES, PBS), pH range 6.5 to 8.5. If the buffer is more concentrated or outside this pH range contact the technical support team.

    Common non-buffering salts (e.g. sodium chloride), chelating agents (e.g. EDTA), and sugars have no effect on conjugation efficiency. Azide (0.02 to 0.1%) and BSA (0.1 to 0.5%) have little or no effect. Glycerol up to 50% has no effect.

    Avoid buffer components that are nucleophilic, as these may react with the kit chemicals. Primary amines (e.g. amino acids or ethanolamine) and thiols (e.g. mercaptoethanol or DTT) fall within this class (Note: Tris-based buffers should be avoided). If your buffer contains primary amines and/or thiols, you should consider using our Concentration and Purification Kits (ab102778 or ab102784).

    Recommended amount and volume of antibody for optimal results

    Pack sizeAmount of antibodyVolume of antibody
    Up to 20 µg10-20 µg4-10 µl
    Up to 200 µg100-200 µg40-100 µl
    Up to 2 mg1-2 mg400-1000 µl

    Antibody concentrations of 1-4 mg/ml generally give optimal results. For conditions outside the suggested ranges contact the technical support team.

    Conjugation reaction

    • Before adding the antibody to the Streptavidin mix, add 1 µl of Modifier reagent to each 10 µl of antibody to be labelled. Mix gently.
    • Remove cap from vial of Streptavidin mix and pipette the antibody sample (with added Modifier) directly onto the lyophilized material. Resuspend gently by withdrawing and re-dispensing the liquid once or twice using a pipette.


    • Replace cap on the vial and leave standing in the dark at room temperature (20-25°C) for 3 hours. Conjugations can also be set up and left overnight; longer incubation times have no negative effect on the conjugate.


    • After incubating for 3 hours (or more), add 1 µl of Quencher reagent for every 10 µl of antibody used and mix gently. The conjugate can be used after 30 minutes. The conjugates do not require purification.


    Storage of conjugates

    Storage at 4°C is recommended for any conjugate. A preservative may be desirable for long-term storage. Other storage conditions may also be satisfactory. The best conditions for any particular conjugate must be determined by experimentation.


    Labeling of the antibody will not work if the conjugation blocks the active paratope. This situation is rare.

  • Tested applications
    Suitable for: Conjugationmore details



Our Abpromise guarantee covers the use of ab102921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.


  • This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.



This product has been referenced in:
  • Lopes FB  et al. Membrane nanoclusters of Fc?RI segregate from inhibitory SIRPa upon activation of human macrophages. J Cell Biol 216:1123-1141 (2017). Read more (PubMed: 28289091) »
  • Zarnegar MA  et al. Targeted chromatin ligation, a robust epigenetic profiling technique for small cell numbers. Nucleic Acids Res 45:e153 (2017). Read more (PubMed: 28973448) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

With regard to the number of biotins, each antibody will be conjugated with multiple biotins, getting up to 6 or 8 biotins per antibody is feasible. It is important to remember that you only need one biotin attac...

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Thank you for your inquiry. I am sorry to hear that ab102921 did not deliver optimal results for you. I recommend to use the kit as instructed with a maximum of 100ul and 100ug of antibody. These kits are optimized for this specific protocol. There is ...

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