Product nameStreptavidin Gold Conjugate (10nm, 10OD)
InnovaCoat® GOLD nanoparticles have a proprietary surface coating that covalently binds antibodies, proteins, peptides or any other biomolecule with free amine groups to form highly stable conjugates. The surface coating can withstand the most extreme conditions (e.g. 2.5 M NaOH at 70°C for 2 hours), making InnovaCoat® GOLD an ideal reagent for a wide range of applications.
Our InnovaCoat® GOLD streptavidin conjugates share the stability attributes of the core nanoparticle. Furthermore, since the streptavidin is covalently bound to the surface coating of the gold nanoparticle it is unable to desorb, which is a major problem faced by competing products whereby the streptavidin is directly attached to the metal. InnovaCoat® GOLD Streptavidin binds with high affinity to biotinylated molecules.
This product is manufactured by Expedeon, an Abcam company. It was previously called 10nm InnovaCoat® GOLD (10OD) Streptavidin Conjugate. Expedeon product code 252-0200 is the same as the 200 µL size of this product and code 252-1000 is the same as the 1 mL size.
InnovaCoat® GOLD Streptavidin is shipped in a buffer composed of TBS containing 0.1% detergent.
If you wish to exchange the InnovaCoat® GOLD Streptavidin into a specific buffer for your assay or test, centrifuge the conjugate at 21,500g at 45 minutes for the 10nm GOLD nanoparticle. Carefully remove the supernatant and add your preferred buffer. It is important to avoid substances that have a very high affinity for gold (e.g. thiols).
The maximum absorbance for the 10nm InnovaCoat®-GOLD Streptavidin is 520nm. To determine the effective concentration of the conjugate obtained we advise to measure the Absmax of light using an UV-vis spectrophotometer after diluting your sample to an appropriate range for your piece of equipment.
The 10nm Streptavidin gold conjugates are more suitable for imaging in electron microscopy. For additional applications please contact us.
Storage instructionsShipped at 4°C. Store at +4°C. Please see notes section.
Storage bufferConstituent: Tris buffered saline
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