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Hello, I was wondering whether there is an antibody pair available for the ELISPOT detection of human CCL1/I-309. As far as I can see nothing is sold as such, but has it been tried or is it known whether maybe a sandwich elisa pair works for this purpose?
Asked on Dec 26 2012
I have confirmed that we do do not have an antibody pair tested for ELISPOT but we do have a pair, ab109788 and ab83413, that have been tested together in sandwich ELISA. Both are rabbit polyclonal antibodies raised against full-length recombinant human I-309, but one is biotinylated, which allows the two rabbit antibodies to be used together in ELISA. Detection is achieved with an avidin or streptavidin enzyme conjugate such as the HRP -conjugated streptavidin ab7403.
Efficacy in ELISPOT is assumed to be likely if efficacy in standard sandwich ELISA has been demonstrated, but optimizing the amount of capture antibody and detection antibody will be necessary.
There are other pairs that might work but that have not been tested together for ELISA of any kind. For example, the mouse monoclonal ab89446 could be used for capture, followed by detection with a polyclonal antibody raised against full-length protein, such as the rabbit anti-I-309 ab109788 or the goat anti-I-309 ab10377, followed by an anti-rabbit IgG or anti-goat IgG secondary conjugate that has been absorbed/purified to remove reactivity with mouse IgG. Please note that our guarantee only applies to tested applications.
Answered on Dec 26 2012