Overview

  • Product name

    Streptavidin HRP (ready-to-use)
  • Tested applications

    Suitable for: IHC-Pmore details
  • General notes

    Streptavidin-HRP for use with biotinylated secondary antibodies during IHC / immunohistochemistry. Optimized, ready-to-use format.

    This product was previously called Streptavidin-Peroxidase.

     

    IHC protocol suitable for use with Streptavidin-HRP ab64269:

    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

    2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.

    3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.

    4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.

    5. Apply primary antibody in antibody diluent and incubate.

    6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.

    7. Apply streptavidin-HRP ab64269 and incubate for 10 minutes at room temperature.

    8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved. Rinse 4 times in buffer.

    9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.

    10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.


    Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Properties

Applications

Our Abpromise guarantee covers the use of ab64269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Images

  • ab64269 Straptavidin Peroxidase used along with an anti-CD20 antibody on Human Tonsil
  • Human colon carcinoma stained with anti-p53 antibody using Streptavidin Peroxidase (ab64269).

References

This product has been referenced in:

  • Petrovic R  et al. Mouse strain and sex as determinants of immune response to trivalent influenza vaccine. Life Sci N/A:N/A (2018). Read more (PubMed: 29859986) »
  • Pokharel A  et al. Asymmetric development of the male mouse mammary gland and its response to a prenatal or postnatal estrogen challenge. Reprod Toxicol 82:63-71 (2018). Read more (PubMed: 30315872) »
See all 6 Publications for this product

Customer reviews and Q&As

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Abreviews
paraffin-embedded mouse/rat mandible/tibia/femur IHC staining에 사용

Miss. myeonga kim

Verified customer

Submitted Jan 24 2018

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