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    streptavidin-hrp-ready-to-use-ab64269.pdf

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Streptavidin HRP (ready-to-use) (ab64269)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)References (36)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin Peroxidase (Ready to Use) (ab64269)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin Peroxidase (Ready to Use) (ab64269)

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Overview

  • Product name

    Streptavidin HRP (ready-to-use)
  • Tested applications

    Suitable for: IHC-Pmore details
  • General notes

    Streptavidin-HRP for use with biotinylated secondary antibodies during IHC / immunohistochemistry. Optimized, ready-to-use format.

    This product was previously called Streptavidin-Peroxidase.

     

    IHC protocol suitable for use with Streptavidin-HRP ab64269:

    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

    2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.

    3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.

    4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.

    5. Apply primary antibody in antibody diluent and incubate.

    6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.

    7. Apply streptavidin-HRP ab64269 and incubate for 10 minutes at room temperature.

    8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved. Rinse 4 times in buffer.

    9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.

    10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.


    Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C.
  • Storage buffer

    pH: 7.60
    Preservative: 0.2% Proclin 300
    Constituent: PBS
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Enzyme
    • Detection
  • Relevance

    Streptavidin peroxidase is used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin Peroxidase (Ready to Use) (ab64269)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin Peroxidase (Ready to Use) (ab64269)
    ab64269 Straptavidin Peroxidase used along with an anti-CD20 antibody on Human Tonsil
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin Peroxidase (Ready to Use) (ab64269)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin Peroxidase (Ready to Use) (ab64269)
    Human colon carcinoma stained with anti-p53 antibody using Streptavidin Peroxidase (ab64269).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (36)

Publishing research using ab64269? Please let us know so that we can cite the reference in this datasheet.

ab64269 has been referenced in 36 publications.

  • Luo L  et al. Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells. Biotechnol J 17:e2100401 (2022). PubMed: 34921593
  • Momin N  et al. Maximizing response to intratumoral immunotherapy in mice by tuning local retention. Nat Commun 13:109 (2022). PubMed: 35013154
  • Badr El-Din NK  et al. Thymax, a gross thymic extract, exerts cell cycle arrest and apoptosis in Ehrlich ascites carcinoma in vivo. Heliyon 8:e09047 (2022). PubMed: 35299600
  • Balint B  et al. Aortic Regurgitation Is Associated With Ascending Aortic Remodeling in the Nondilated Aorta. Arterioscler Thromb Vasc Biol 41:1179-1190 (2021). PubMed: 33441026
  • Al-Shabebi A  et al. Localization and expression of ADAM2 in the dromedary camel testis, epididymis and sperm during rutting season. Anim Reprod 18:e20200241 (2021). PubMed: 33936295
View all Publications for this product

Customer reviews and Q&As

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ab64269 - Streptavidin Peroxidase (Ready to Use)

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
paraffin-embedded mouse/rat mandible/tibia/femur IHC staining에 사용
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. myeonga kim

Verified customer

Submitted Jan 24 2018

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