Product nameStreptavidin Peroxidase (Ready to Use)
Tested applicationsSuitable for: IHC-P, Sandwich ELISAmore details
Streptavidin-horseradish peroxidase (HRP) for use in labeled streptavidin-biotin (LSAB) detection during IHC. Ready-to-use format.
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Add enough drops of an appropriate Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
3. Perform appropriate pretreatment if required. Wash 3 times in buffer.
4. Apply an appropriate Protein Block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
5. Apply primary antibody and incubate according to manufacturer's protocol.
6. Wash 4 times in buffer. Incubate slide with biotinylated secondary solution according to manufacturer’s protocol. Wash 4 times in buffer.
7. Apply Streptavidin Peroxidase and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
9. Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.
Storage instructionsStore at +4°C.
Storage bufferpH: 7.60
Preservative: 0.2% Proclin
Concentration information loading...
RelevanceStreptavidin peroxidase is used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Our Abpromise guarantee covers the use of ab64269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|Sandwich ELISA||Use at an assay dependent dilution. PubMed: 21371998|
This product has been referenced in:
- Petrovic R et al. Mouse strain and sex as determinants of immune response to trivalent influenza vaccine. Life Sci N/A:N/A (2018). Read more (PubMed: 29859986) »
- Pokharel A et al. Asymmetric development of the male mouse mammary gland and its response to a prenatal or postnatal estrogen challenge. Reprod Toxicol 82:63-71 (2018). Read more (PubMed: 30315872) »