Overview

  • Product name
    Streptavidin Peroxidase (Ready to Use)
  • Tested applications
    Suitable for: IHC-P, Sandwich ELISAmore details
  • General notes

    Streptavidin-horseradish peroxidase (HRP) for use in labeled streptavidin-biotin (LSAB) detection during IHC. Ready-to-use format.

     

    STAINING PROTOCOL:

    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

    2. Add enough drops of an appropriate Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.

    3. Perform appropriate pretreatment if required. Wash 3 times in buffer.

    4. Apply an appropriate Protein Block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.

    5. Apply primary antibody and incubate according to manufacturer's protocol.

    6. Wash 4 times in buffer. Incubate slide with biotinylated secondary solution according to manufacturer’s protocol. Wash 4 times in buffer.

    7. Apply Streptavidin Peroxidase and incubate for 10 minutes at room temperature.

    8. Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.

    9. Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.

    10. Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.

Properties

Applications

Our Abpromise guarantee covers the use of ab64269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. PubMed: 21371998

Images

  • ab64269 Straptavidin Peroxidase used along with an anti-CD20 antibody on Human Tonsil
  • Human colon carcinoma stained with anti-p53 antibody using Streptavidin Peroxidase (ab64269).

References

This product has been referenced in:
  • Petrovic R  et al. Mouse strain and sex as determinants of immune response to trivalent influenza vaccine. Life Sci N/A:N/A (2018). Read more (PubMed: 29859986) »
  • Pokharel A  et al. Asymmetric development of the male mouse mammary gland and its response to a prenatal or postnatal estrogen challenge. Reprod Toxicol 82:63-71 (2018). Read more (PubMed: 30315872) »
See all 6 Publications for this product

Customer reviews and Q&As

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Abreviews
paraffin-embedded mouse/rat mandible/tibia/femur IHC staining에 사용

Miss. myeonga kim

Verified customer

Submitted Jan 24 2018

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