Streptavidin-Phycoerythrin (R-PE) (ab239759)
Key features and details
- Expression system: Escherichia coli
- Suitable for: Flow Cyt
Description
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Product name
Streptavidin-Phycoerythrin (R-PE)
See all Streptavidin proteins and peptides -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Streptomyces avidinii
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Conjugation
PE. Ex: 488nm, Em: 575nm -
Description
Recombinant Streptavidin protein (PE)
Specifications
Our Abpromise guarantee covers the use of ab239759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Flow Cytometry
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Form
Liquid -
Additional notes
Streptavidin-PE will bind to biotin allowing detection of biotinylated antibodies in Flow, microplate assays and microarrays. Streptavidin high affinity for biotin allows low background in cell sorting, immunofluorescence, in situ hybridization, or flow cytometry. Suitable for use with Mouse, Human and Rat samples.
Excitation: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C. Store In the Dark.
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 2.9% Sodium chloride
General Info
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Alternative names
- SA V1
- SA V2
- strepavidin
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Relevance
Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. -
Cellular localization
Cytoplasmic
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab239759 has not yet been referenced specifically in any publications.