Description

  • Product name

    Streptavidin-Phycoerythrin (R-PE)
    See all Streptavidin proteins and peptides
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Streptomyces avidinii
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Description

    Recombinant Streptavidin protein (Phycoerythrin)

Specifications

Our Abpromise guarantee covers the use of ab239759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Flow Cytometry

  • Form

    Liquid
  • Additional notes

    Streptavidin-PE will bind to biotin allowing detection of biotinylated antibodies in Flow, microplate assays and microarrays. Streptavidin high affinity for biotin allows low background in cell sorting, immunofluorescence, in situ hybridization, or flow cytometry. Suitable for use with Mouse, Human and Rat samples.

    Excitation: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Store In the Dark.

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 2.9% Sodium chloride

General Info

  • Alternative names

    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • Relevance

    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • Cellular localization

    Cytoplasmic

Images

  • C57Bl/6 splenocytes were stained with Biotin Anti-Mouse CD19 (solid line) or Biotin Rat IgG2a isotype control (dashed line), followed by 0.06 µg ab239759.

References

ab239759 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab239759.
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