Overview

  • Product name

    Anti-Streptococcus Group B antibody
    See all Streptococcus Group B primary antibodies
  • Description

    Rabbit polyclonal to Streptococcus Group B
  • Host species

    Rabbit
  • Specificity

    Reacts with type specific carbohydrate of group B Streptococcus.
  • Tested applications

    Suitable for: IHC-P, ELISA, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Native Streptococcus Group B.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab53584 was affinity purified.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Streptococci form part of the normal human flora that resides on the skin, and can also colonise the respiratory, gastrointestinal, and genitourinary tracts. Streptococci can cause a range of diseases, from the less serious but common sore throats and skin infections to life threatening conditions such as necrotising fasciitis. Different streptococcal species are involved in human disease, broadly categorised as pus forming or pyogenic streptococci, non pus forming or non pyogenic streptococci, and Streptococcus pneumoniae. Streptococci are classified into Lancefield serotypes by their cell wall polysaccharide antigens. Group A are primarily pathogens. Group B streptococci (including Streptococcus agalactiae) are the leading bacterial causes of human neonatal illness and death causing opportunistic invasive disease in pregnant women such as preterm labour, membrane rupture and urinary tract infections and sepsis and meningitis in newborns.
  • Alternative names

    • Beta strep antibody
    • Group b streptococci antibody
    • Group b streptococcus antibody
    • Strep b antibody

Images

  • ab53584 staining Streptococcus agalactiae by Immunocytochemistry/ Immunofluorescence.
    Streptococcus agalactiae was infected into fetal rat lung explants for 24 hours. Tissue was fixed and probed "whole-mount". The cells were formaldehyde fixed, permeabilised in 0.1% Triton/1X PBS for 15 minutes and then blocked using 5% Donkey serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for one hour at 25°C. The secondary antibody used was a Donkey anti-rabbit IgG conjugated to FITC used at a 1/400 dilution. Tissue was counterstained with Hoechst 33342 (blue). Image collected using a Leica Microsystems confocal microscope.

    See Abreview

  • ab53584 staining Streptococcus Group B in brain tissue from Oreochromis niloticus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in 10% buffered formalin and a heat mediated antigen retrieval step was performed using citrate. Samples were then blocked with 25% serum for 30 minutes at room temperature and then incubated with ab53584 at a 1/200 dilution for 18 hours at 4°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

References

This product has been referenced in:

  • Six A  et al. Galleria mellonella as an infection model for the multi-host pathogen Streptococcus agalactiae reflects hypervirulence of strains associated with human invasive disease. Virulence 10:600-609 (2019). Read more (PubMed: 31230520) »
  • Randis TM  et al. Group B Streptococcus ß-hemolysin/Cytolysin Breaches Maternal-Fetal Barriers to Cause Preterm Birth and Intrauterine Fetal Demise in Vivo. J Infect Dis N/A:N/A (2014). Read more (PubMed: 24474814) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

ab53584 has been testedin immunocytochemistry (ICC) but not in colloidal gold method.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Fish Tissue sections (Brain from tilapia (Oreochromis niloticus))
Specification
Brain from tilapia (Oreochromis niloticus)
Fixative
10% buffered formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 25% · Temperature: RT°C

Christian Delannoy

Verified customer

Submitted Feb 02 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Streptococcus agalactiae Cell
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton/1X PBS for 15 minutes
Blocking step
Donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. David Kling

Verified customer

Submitted Sep 16 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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