Key features and details
- Rabbit polyclonal to Streptococcus Group B
- Suitable for: IHC-P, ELISA, IHC-Fr, ICC/IF
- Isotype: IgG
Product nameAnti-Streptococcus Group B antibody
See all Streptococcus Group B primary antibodies
DescriptionRabbit polyclonal to Streptococcus Group B
SpecificityReacts with type specific carbohydrate of group B Streptococcus.
Tested applicationsSuitable for: IHC-P, ELISA, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Other species
Native Streptococcus Group B.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab53584 was affinity purified.
Our Abpromise guarantee covers the use of ab53584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceStreptococci form part of the normal human flora that resides on the skin, and can also colonise the respiratory, gastrointestinal, and genitourinary tracts. Streptococci can cause a range of diseases, from the less serious but common sore throats and skin infections to life threatening conditions such as necrotising fasciitis. Different streptococcal species are involved in human disease, broadly categorised as pus forming or pyogenic streptococci, non pus forming or non pyogenic streptococci, and Streptococcus pneumoniae. Streptococci are classified into Lancefield serotypes by their cell wall polysaccharide antigens. Group A are primarily pathogens. Group B streptococci (including Streptococcus agalactiae) are the leading bacterial causes of human neonatal illness and death causing opportunistic invasive disease in pregnant women such as preterm labour, membrane rupture and urinary tract infections and sepsis and meningitis in newborns.
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ab53584 staining Streptococcus agalactiae by Immunocytochemistry/ Immunofluorescence.
Streptococcus agalactiae was infected into fetal rat lung explants for 24 hours. Tissue was fixed and probed "whole-mount". The cells were formaldehyde fixed, permeabilised in 0.1% Triton/1X PBS for 15 minutes and then blocked using 5% Donkey serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for one hour at 25°C. The secondary antibody used was a Donkey anti-rabbit IgG conjugated to FITC used at a 1/400 dilution. Tissue was counterstained with Hoechst 33342 (blue). Image collected using a Leica Microsystems confocal microscope.
ab53584 staining Streptococcus Group B in brain tissue from Oreochromis niloticus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in 10% buffered formalin and a heat mediated antigen retrieval step was performed using citrate. Samples were then blocked with 25% serum for 30 minutes at room temperature and then incubated with ab53584 at a 1/200 dilution for 18 hours at 4°C. The secondary used was an undiluted HRP conjugated goat polyclonal.
ab53584 has been referenced in 4 publications.
- Six A et al. Galleria mellonella as an infection model for the multi-host pathogen Streptococcus agalactiae reflects hypervirulence of strains associated with human invasive disease. Virulence 10:600-609 (2019). PubMed: 31230520
- Ding T et al. Sex-Dependent Influence of Developmental Toxicant Exposure on Group B Streptococcus-Mediated Preterm Birth in a Murine Model. Reprod Sci 25:662-673 (2018). PubMed: 29153057
- Randis TM et al. Group B Streptococcus ß-hemolysin/Cytolysin Breaches Maternal-Fetal Barriers to Cause Preterm Birth and Intrauterine Fetal Demise in Vivo. J Infect Dis N/A:N/A (2014). PubMed: 24474814
- Kling DE et al. Group B Streptococcus induces a caspase-dependent apoptosis in fetal rat lung interstitium. Microb Pathog 61-62C:1-10 (2013). PubMed: 23624260