Overview

  • Product name
    Anti-Streptococcus pyogenes Group A Carbohydrate antibody
  • Description
    Goat polyclonal to Streptococcus pyogenes Group A Carbohydrate
  • Host species
    Goat
  • Specificity
    Streptococcus Pyogenes Group A
  • Tested applications
    Suitable for: Dot blot, ELISA, ICC/IF, IHC-Fr, IP, WB, IHC-Pmore details
  • Immunogen

    Goats were intensely immunized, intravenously, with killed Streptococcus pyogenes vaccine Strain J17A4.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Serum was collected and antibody to the Group A carbohydrate was immunoaffinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
ELISA
ICC/IF
IHC-Fr
IP
WB
IHC-P
  • Application notes
    This antibody is suitable for colloidal gold conjugation
  • Target

    • Relevance
      Streptococcus pyogenes (Group A streptococcus) is a Gram-positive, nonmotile, nonsporeforming coccus that occurs in chains or in pairs of cells. Individual cells are round-to-ovoid cocci, 0.6-1.0 micrometer in diameter. Streptococci divide in one plane and thus occur in pairs or (especially in liquid media or clinical material) in chains of varying lengths. The metabolism of S. pyogenes is fermentative; the organism is a catalase-negative aerotolerant anaerobe (facultative anaerobe), and requires enriched medium containing blood in order to grow. Group A streptococci typically have a capsule composed of hyaluronic acid and exhibit beta (clear) hemolysis on blood agar.

    References

    This product has been referenced in:
    • Sumitomo T  et al. Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection. Front Cell Infect Microbiol 8:10 (2018). IHC-P . Read more (PubMed: 29416987) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your recent telephone enquiry.

    I am sorry it has taken some time ot obtain the required information for you as I had quite a few people to contact to gather the details.

    I obtained information on the following Strep group A antibodies that have been tested in western blot. As discussed on the telephone, I am sorry to confirm that these will not have been tested in vivo.

    ab20509 Positive for Group A (ATCC 10403), Negative for Group B, Group C, Group D, Group F, Group G, S. aureus, Treponema reiter, Neisseria perf., Streptococcus pneumonia mix (8 strains), Bacillus cereus and human cells.
    https://www.abcam.com/index.html?datasheet=20509 (or use the following: https://www.abcam.com/index.html?datasheet=20509).

    ab20752 Positive for Group A (ATCC 10403), Negative for Group B, Group C, Group D, Group F, Group G, S. aureus, Treponema reiter, Neisseria perf., Streptococcus pneumonia mix (8 strains), Bacillus cereus and human cells.
    https://www.abcam.com/index.html?datasheet=20752 (or use the following: https://www.abcam.com/index.html?datasheet=20752).

    ab20915 Positive for Streptococcus pyogenes (ATCC 12344, UV-inactivated), Negative for Group B: Type 1a (ATCC 12400), Type 1b (ATCC 12401), Type 1c (ATCC 27591), Type II (ATCC 12973) and Type III (ATCC 12403); Group C = ATCC 12960; Group D = ATCC 12389; Group E = ATCC 12390, Group F = ATCC 12393 & Group G = ATCC 27961.
    https://www.abcam.com/index.html?datasheet=20915 (or use the following: https://www.abcam.com/index.html?datasheet=20915).


    ab21034 Positive for Group A (ATCC 10403), Negative for Group B, Group C, Group D, Group F, Group G, S. aureus, Treponema reiter, Neisseria perf., Streptococcus pneumonia mix (8 strains), Bacillus cereus and human cells.
    https://www.abcam.com/index.html?datasheet=21034 (or use the following: https://www.abcam.com/index.html?datasheet=21034).

    ab21046 Positive for Group A (ATCC 10403), Negative for Group B, Group C, Group D, Group F, Group G, S. aureus, Treponema reiter, Neisseria perf., Streptococcus pneumonia mix (8 strains), Bacillus cereus and human cells.
    https://www.abcam.com/index.html?datasheet=21046 (or use the following: https://www.abcam.com/index.html?datasheet=21046).

    ab25870 Specific for Group A carbohydrate.
    https://www.abcam.com/index.html?datasheet=25870 (or use the following: https://www.abcam.com/index.html?datasheet=25870).


    ab36303 Anti-Streptococcus group A antibody [5A1]
    https://www.abcam.com/index.html?datasheet=36303 (or use the following: https://www.abcam.com/index.html?datasheet=36303).
    This antibody was tested and found positive against the following:
    a. Group A Streptococcus = Streptococcus pyogenes, ATCC #12344

    2. The antibody was tested and found negative against the following:

    a. Group B Streptococcus = Type Ia (ATCC # 12400), Type Ib (ATCC # 12401), Type Ic (ATCC #27591), Type II (ATCC # 12973), and Type III (ATCC# 12403)

    b. Group C Streptococcus = ATCC # 12960

    c. Group D Streptococcus = ATCC #12389

    d. Group E Streptococcus = ATCC # 12390

    e. Group F Streptococcus = ATCC #12393

    f. Group G Streptococcus = ATCC #27961


    ab68879 ab68880 ab68881 have been specifically tested for reactivity against the ATCC 10403 strain of Group A Streptococcus. It was also tested and found to be negative against Treponema ryder, S. pneumoniae, and Streptococcus Group B, C, D, F, and G.
    https://www.abcam.com/index.html?datasheet=68879 (or use the following: https://www.abcam.com/index.html?datasheet=68879).
    https://www.abcam.com/index.html?datasheet=68880 (or use the following: https://www.abcam.com/index.html?datasheet=68880).
    https://www.abcam.com/index.html?datasheet=68881 (or use the following: https://www.abcam.com/index.html?datasheet=68881).

    I hope this information will be helpful. If you have any further questions, please do not hesitate to contact me.

    Read More

    Answer

    We would recommend a starting dilution of 1:200.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up