The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000 - 1/5000. In a simple ELISA with 0.5µg Streptokinase coated per well, the 50% OD was observed at an antibody dilution of 1/2500. Under the same conditions the end point was observed at 1/50000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Streptokinase is a metallo-enzyme produced by various strains of Streptococcus that can liquify blood clots by converting plasminogen to plasmin. Streptokinase is not a protease but it activates plasminogen by complexing with it. It is thought to prevent the formation of effective fibrin barriers around the site of infection, thereby contributing to the invasiveness of the cells.
This product has been referenced in:
Kinnby B et al. Plasminogen binding by oral streptococci from dental plaque and inflammatory lesions. Microbiology154:924-31 (2008).
Read more (PubMed: 18310038) »