• Product name

    Anti-Streptolysin O (Hemolytic streptococcus) antibody
  • Description

    Rabbit polyclonal to Streptolysin O (Hemolytic streptococcus)
  • Host species

  • Specificity

    ab188539 reacts with Streptolysin O of Group A, C and G origins.
  • Tested applications

    Suitable for: WB, Neutralising, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Streptolysin O (Hemolytic streptococcus). (from S. dysgalactiae subsp. equismilis H46A (group C hemolytic Streptococci) expressed and highly purified from E. coli).



Our Abpromise guarantee covers the use of ab188539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
Neutralising Use at an assay dependent concentration.

Neutralization of hemolysis by Streptolysin O (SLO).

IP Use at an assay dependent concentration.


  • Relevance

    Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cell. SLO is oxygen-sensitive and is easily inactivated in its presence but can be reactivated by thiol compounds , so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.
  • Alternative names

    • Streptolysin O (Thiol-activated cytolysin) slo antibody
    • Streptolysin O antibody


  • All lanes : Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution

    Lane 1 : Medium only
    Lane 2 : Culture supernatant of GAS strain Sa
    Lane 3 : Culture supernatant of GCS strain H46A

    Predicted band size: 64 kDa

    Most of Streptolysin O in culture medium of GAS strain Sa is proteolytically degraded, accounting for low activity level of Streptolysin O in the culture of this strain. Condensed sample is analyzed in the second Western Blot.


    The two faint bands below Streptolysin O band are partially degraded Streptolysin O.

  • All lanes : Anti-Streptolysin O (Hemolytic streptococcus) antibody (ab188539) at 1/2000 dilution

    Lane 1 : Concentrated culture of GSA strain Sa
    Lane 2 : Concentrated medium only

    Predicted band size: 64 kDa

    Culture supernatant was approximately 1,000~2,000 fold concentrated from culture medium by ammonium fractionation and heat-precipitation.


    The Streptolysin O of GSA strains is degraded by an exoprotease called streptococcal pyrogenic exotoxin B and both the concentration and size of Streptolysin O in culture medium is decreased from the native one.


This product has been referenced in:

  • Hancz D  et al. Inhibition of Inflammasome-Dependent Interleukin 1ß Production by Streptococcal NAD(+)-Glycohydrolase: Evidence for Extracellular Activity. MBio 8:N/A (2017). Read more (PubMed: 28720729) »
See 1 Publication for this product

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