Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11801] to Striatin 4
- Suitable for: WB, Flow Cyt, ICC/IF, IP
- Reacts with: Human
Product nameAnti-Striatin 4 antibody [EPR11801]
See all Striatin 4 primary antibodies
DescriptionRabbit monoclonal [EPR11801] to Striatin 4
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Striatin 4.
Database link: Q9NRL3
- Human fetal brain, fetal lung, and Human cerebellum lysates. K562 and U87-MG cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
FunctionBinds calmodulin in a calcium dependent manner. May function as scaffolding or signaling protein.
Sequence similaritiesBelongs to the WD repeat striatin family.
Contains 7 WD repeats.
Cellular localizationCytoplasm. Membrane. Cell projection, dendritic spine. CTTNBP2-binding may regulate dendritic spine distribution.
- Information by UniProt
- PPP2R6C antibody
- Protein phosphatase 2 regulatory subunit B'''gamma antibody
- Striatin, calmodulin binding protein 4 antibody
All lanes : Anti-Striatin 4 antibody [EPR11801] (ab177155) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : K562 lysate
Lane 3 : U87-MG lysate
Lane 4 : Human cerebellum lysate
Lane 5 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa
Immunofluorescent analysis of U87-MG cells labeling Striatin 4 with ab177155 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from Human fetal brain lysate, immunoprecipitated using ab177155 at 1/10 dilution.
Flow cytometric analysis of permeabilized K562 cells labeling Striatin 4 with ab177155 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
ab177155 has been referenced in 1 publication.
- Kim JW et al. STRIPAK directs PP2A activity toward MAP4K4 to promote oncogenic transformation of human cells. Elife 9:N/A (2020). PubMed: 31913126