Anti-Stromal interaction molecule 1 antibody (ab240592)
Key features and details
- Rabbit polyclonal to Stromal interaction molecule 1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Stromal interaction molecule 1 antibody
See all Stromal interaction molecule 1 primary antibodies -
Description
Rabbit polyclonal to Stromal interaction molecule 1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide within Human Stromal interaction molecule 1 aa 635-685. The exact sequence is proprietary. (NP_003147.2)
Database link: Q13586 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab240592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/500 - 1/2500. Predicted molecular weight: 77 kDa. |
Target
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Function
Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. -
Tissue specificity
Ubiquitously expressed in various human primary cells and tumor cell lines. -
Involvement in disease
Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
Post-translational
modificationsGlycosylation is required for cell surface expression.
Phosphorylated predominantly on Ser residues. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules. - Information by UniProt
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Database links
- Entrez Gene: 534816 Cow
- Entrez Gene: 6786 Human
- Entrez Gene: 20866 Mouse
- Entrez Gene: 361618 Rat
- Omim: 605921 Human
- SwissProt: Q58CP9 Cow
- SwissProt: Q13586 Human
- SwissProt: P70302 Mouse
see all -
Alternative names
- D11S4896E antibody
- GOK antibody
- OTTHUMP00000164512 antibody
see all
Images
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Western blot - Anti-Stromal interaction molecule 1 antibody (ab240592)All lanes : Anti-Stromal interaction molecule 1 antibody (ab240592) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Predicted band size: 77 kDaDetection: Chemiluminescence with exposure time of 3 minutes
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Immunoprecipitation - Anti-Stromal interaction molecule 1 antibody (ab240592)Stromal interaction molecule 1 was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab240592 at 6 μg/mL lysate. Western Blot was performed on the immunoprecipitate using ab240592 at 1 μg/mL.
Lane 1: ab240592 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with exposure times of 3 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240592 has not yet been referenced specifically in any publications.
