Key features and details
- Rabbit polyclonal to Stromal interaction molecule 1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Stromal interaction molecule 1 antibody
See all Stromal interaction molecule 1 primary antibodies
DescriptionRabbit polyclonal to Stromal interaction molecule 1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide within Human Stromal interaction molecule 1 aa 635-685. The exact sequence is proprietary. (NP_003147.2)
Database link: Q13586
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
Our Abpromise guarantee covers the use of ab240592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/500 - 1/2500. Predicted molecular weight: 77 kDa.|
FunctionPlays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
Tissue specificityUbiquitously expressed in various human primary cells and tumor cell lines.
Involvement in diseaseDefects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
Sequence similaritiesContains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain.
DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
modificationsGlycosylation is required for cell surface expression.
Phosphorylated predominantly on Ser residues.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
- Information by UniProt
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All lanes : Anti-Stromal interaction molecule 1 antibody (ab240592) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Predicted band size: 77 kDa
Detection: Chemiluminescence with exposure time of 3 minutes
Stromal interaction molecule 1 was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab240592 at 6 μg/mL lysate. Western Blot was performed on the immunoprecipitate using ab240592 at 1 μg/mL.
Lane 1: ab240592 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with exposure times of 3 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240592 has not yet been referenced specifically in any publications.