Overview

  • Product name
    Anti-Stromal interaction molecule 1 antibody
    See all Stromal interaction molecule 1 primary antibodies
  • Description
    Mouse monoclonal to Stromal interaction molecule 1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 24-686 of Human Stromal interaction molecule 1

Properties

Applications

Our Abpromise guarantee covers the use of ab57834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 77 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 10 µg/ml.
IP Use at 4 µg/mg of lysate.

Target

  • Function
    Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
  • Tissue specificity
    Ubiquitously expressed in various human primary cells and tumor cell lines.
  • Involvement in disease
    Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
  • Sequence similarities
    Contains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Domain
    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    modifications
    Glycosylation is required for cell surface expression.
    Phosphorylated predominantly on Ser residues.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • D11S4896E antibody
    • GOK antibody
    • OTTHUMP00000164512 antibody
    • OTTHUMP00000229140 antibody
    • OTTHUMP00000230742 antibody
    • SIM antibody
    • STIM 1 antibody
    • STIM1 antibody
    • Stim1 stromal interaction molecule 1 antibody
    • STIM1_HUMAN antibody
    • STIM1L antibody
    • Stromal interaction molecule 1 antibody
    see all

Images

  • Stromal interaction molecule 1 antibody (ab57834) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Stromal interaction molecule 1 antibody (ab57834) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B.
  • ab57834 at 10 ug/ml staining Stromal interaction molecule 1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • ab57834 staining the stromal interaction molecule in HeLa cells by immunocytochemistry/immunofluorescence (ICC/IF). Cells were fixed with paraformaldehyde and permeabilized with 0.5% TritonX. Samples were incubated with primary antibody (1/200) for 1 hour at 24°C. An Alexa Fluor ® 488-conjugated chicken anti-mouse polyclonal (1/1000) was used as the secondary.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab57834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57834, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Zhang W  et al. Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements Facilitates Analysis of Biological Endpoint Effects of Cellular Ca2+ Mobilization. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29463029) »
  • Li P  et al. Blocking of stromal interaction molecule 1 expression influence cell proliferation and promote cell apoptosis in vitro and inhibit tumor growth in vivo in head and neck squamous cell carcinoma. PLoS One 12:e0177484 (2017). WB . Read more (PubMed: 28494008) »
See all 5 Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
1000 µg
Specification
HeLa
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Oct 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
40 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - other (HELA)
Total protein in input
200 µg
Specification
HELA
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Sep 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X

Abcam user community

Verified customer

Submitted Aug 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HeLa)
Loading amount
50 µg
Specification
HeLa
Treatment
Control and STIM1 siRNA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Aug 18 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (egg extract)
Loading amount
15 µg
Specification
egg extract
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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