Overview

  • Product name

    Anti-Stromal interaction molecule 1 antibody
    See all Stromal interaction molecule 1 primary antibodies
  • Description

    Rabbit polyclonal to Stromal interaction molecule 1
  • Host species

    Rabbit
  • Specificity

    ab62031 detects the larger isoform of Stromal interaction molecule 1 only. It is predicted to have no cross-reactivity with Stromal interaction molecule 2.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Raised against a 24 amino acid synthetic peptide from near the carboxy terminus of Human Stromal interaction molecule 1 (GenBank accession no. NP_003147).

  • Positive control

    • Mouse thymus tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab62031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function

    Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
  • Tissue specificity

    Ubiquitously expressed in various human primary cells and tumor cell lines.
  • Involvement in disease

    Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
  • Sequence similarities

    Contains 1 EF-hand domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Domain

    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    modifications

    Glycosylation is required for cell surface expression.
    Phosphorylated predominantly on Ser residues.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • D11S4896E antibody
    • GOK antibody
    • OTTHUMP00000164512 antibody
    • OTTHUMP00000229140 antibody
    • OTTHUMP00000230742 antibody
    • SIM antibody
    • STIM 1 antibody
    • STIM1 antibody
    • Stim1 stromal interaction molecule 1 antibody
    • STIM1_HUMAN antibody
    • STIM1L antibody
    • Stromal interaction molecule 1 antibody
    see all

Images

  • Lane 1 : Anti-Stromal interaction molecule 1 antibody (ab62031) at 1 µg/ml
    Lane 2 : Anti-Stromal interaction molecule 1 antibody (ab62031) at 2 µg/ml

    All lanes : Mouse thymus tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 77 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 116 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse spleen tissue labeling Stromal interaction molecule 1 with Anti-Stromal interaction molecule 1 antibody (ab62031) at 5μg/ml.
  • Immunofluorescence of Stromal interaction molecule 1 in mouse spleen cells with ab62031 at 20 μg/mL.

  • Immunofluorescence of Stromal interaction molecule 1 in Hek293 cells with ab62031 at 20 μg/mL.

  • ab62031 at 2.5 µg/ml staining Stromal interaction molecule 1 in human spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

References

This product has been referenced in:

  • Sathish V  et al. Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle. Cell Physiol Biochem 36:1101-15 (2015). Read more (PubMed: 26113109) »
  • Vohra PK  et al. TRPC3 regulates release of brain-derived neurotrophic factor from human airway smooth muscle. Biochim Biophys Acta 1833:2953-60 (2013). Read more (PubMed: 23899746) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (B and T lymphocytes)
Permeabilization
Yes - Triton / MeOH
Specification
B and T lymphocytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde

Ms. Anna Kabanova

Verified customer

Submitted Mar 28 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Lung)
Loading amount
25 µg
Specification
Lung
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 29 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up