Key features and details
- Rabbit polyclonal to Stromal interaction molecule 1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Stromal interaction molecule 1 antibody
See all Stromal interaction molecule 1 primary antibodies
DescriptionRabbit polyclonal to Stromal interaction molecule 1
Specificityab62031 detects the larger isoform of Stromal interaction molecule 1 only. It is predicted to have no cross-reactivity with Stromal interaction molecule 2.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Raised against a 24 amino acid synthetic peptide from near the carboxy terminus of Human Stromal interaction molecule 1 (GenBank accession no. NP_003147).
- Mouse thymus tissue lysate
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).|
|IHC-P||Use a concentration of 2.5 µg/ml.|
FunctionPlays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
Tissue specificityUbiquitously expressed in various human primary cells and tumor cell lines.
Involvement in diseaseDefects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
Sequence similaritiesContains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain.
DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
modificationsGlycosylation is required for cell surface expression.
Phosphorylated predominantly on Ser residues.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
- Information by UniProt
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Lane 1 : Anti-Stromal interaction molecule 1 antibody (ab62031) at 1 µg/ml
Lane 2 : Anti-Stromal interaction molecule 1 antibody (ab62031) at 2 µg/ml
All lanes : Mouse thymus tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 77 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 116 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse spleen tissue labeling Stromal interaction molecule 1 with Anti-Stromal interaction molecule 1 antibody (ab62031) at 5μg/ml.
Immunofluorescence of Stromal interaction molecule 1 in mouse spleen cells with ab62031 at 20 μg/mL.
Immunofluorescence of Stromal interaction molecule 1 in Hek293 cells with ab62031 at 20 μg/mL.
ab62031 at 2.5 µg/ml staining Stromal interaction molecule 1 in human spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
ab62031 has been referenced in 3 publications.
- Sathish V et al. Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle. Cell Physiol Biochem 36:1101-15 (2015). PubMed: 26113109
- Vohra PK et al. TRPC3 regulates release of brain-derived neurotrophic factor from human airway smooth muscle. Biochim Biophys Acta 1833:2953-60 (2013). PubMed: 23899746
- Abcejo AJ et al. Brain-derived neurotrophic factor enhances calcium regulatory mechanisms in human airway smooth muscle. PLoS One 7:e44343 (2012). WB . PubMed: 22952960