Anti-Stromal interaction molecule 1 antibody (ab62031)
Key features and details
- Rabbit polyclonal to Stromal interaction molecule 1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Stromal interaction molecule 1 antibody
See all Stromal interaction molecule 1 primary antibodies -
Description
Rabbit polyclonal to Stromal interaction molecule 1 -
Host species
Rabbit -
Specificity
ab62031 detects the larger isoform of Stromal interaction molecule 1 only. It is predicted to have no cross-reactivity with Stromal interaction molecule 2. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Raised against a 24 amino acid synthetic peptide from near the carboxy terminus of Human Stromal interaction molecule 1 (GenBank accession no. NP_003147).
-
Positive control
- Mouse thymus tissue lysate
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab62031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa). | |
| IHC-P | Use a concentration of 2.5 µg/ml. |
Target
-
Function
Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. -
Tissue specificity
Ubiquitously expressed in various human primary cells and tumor cell lines. -
Involvement in disease
Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
Post-translational
modificationsGlycosylation is required for cell surface expression.
Phosphorylated predominantly on Ser residues. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules. - Information by UniProt
-
Database links
- Entrez Gene: 6786 Human
- Entrez Gene: 20866 Mouse
- Entrez Gene: 361618 Rat
- Omim: 605921 Human
- SwissProt: Q13586 Human
- SwissProt: P70302 Mouse
- SwissProt: P84903 Rat
- Unigene: 501735 Human
see all -
Alternative names
- D11S4896E antibody
- GOK antibody
- OTTHUMP00000164512 antibody
see all
Images
-
Western blot - Anti-Stromal interaction molecule 1 antibody (ab62031)Lane 1 : Anti-Stromal interaction molecule 1 antibody (ab62031) at 1 µg/ml
Lane 2 : Anti-Stromal interaction molecule 1 antibody (ab62031) at 2 µg/ml
All lanes : Mouse thymus tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 77 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 116 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody (ab62031)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse spleen tissue labeling Stromal interaction molecule 1 with Anti-Stromal interaction molecule 1 antibody (ab62031) at 5μg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Stromal interaction molecule 1 antibody (ab62031)Immunofluorescence of Stromal interaction molecule 1 in mouse spleen cells with ab62031 at 20 μg/mL.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody (ab62031)Immunofluorescence of Stromal interaction molecule 1 in Hek293 cells with ab62031 at 20 μg/mL.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody (ab62031)ab62031 at 2.5 µg/ml staining Stromal interaction molecule 1 in human spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Protocols
Datasheets and documents
References (3)
ab62031 has been referenced in 3 publications.
- Sathish V et al. Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle. Cell Physiol Biochem 36:1101-15 (2015). PubMed: 26113109
- Vohra PK et al. TRPC3 regulates release of brain-derived neurotrophic factor from human airway smooth muscle. Biochim Biophys Acta 1833:2953-60 (2013). PubMed: 23899746
- Abcejo AJ et al. Brain-derived neurotrophic factor enhances calcium regulatory mechanisms in human airway smooth muscle. PLoS One 7:e44343 (2012). WB . PubMed: 22952960
