Antigen retrieval with citrate buffer (pH 6): Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
Is unsuitable for Flow Cyt or ICC.
Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
Ubiquitously expressed in various human primary cells and tumor cell lines.
Involvement in disease
Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
Glycosylation is required for cell surface expression. Phosphorylated predominantly on Ser residues.
Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
Yang S et al. Store operated calcium channels are associated with diabetic cystopathy in streptozotocin-induced diabetic rats. Mol Med Rep17:6612-6620 (2018).
Read more (PubMed: 29532875) »
Wu G et al. Overexpression of stromal interaction molecule 1 may promote epithelial-mesenchymal transition and indicate poor prognosis in gastric cancer. Mol Med Rep16:151-158 (2017).
Read more (PubMed: 28534934) »