Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994)


  • Product name
    Anti-Stromal interaction molecule 1 antibody [EPR3414]
    See all Stromal interaction molecule 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue, cells or virus within Human Stromal interaction molecule 1 aa 650-750. The exact sequence is proprietary.

  • Positive control
    • SH SY5Y, K562, HepG2, HeLa and LnCaP cell lysates; Human breast tissue.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100.

Antigen retrieval with citrate buffer (pH 6): Heat up to 98 &degC, below boiling, and then let cool for 10-20 min.

  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
    • Tissue specificity
      Ubiquitously expressed in various human primary cells and tumor cell lines.
    • Involvement in disease
      Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
    • Sequence similarities
      Contains 1 EF-hand domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Domain
      The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
    • Post-translational
      Glycosylation is required for cell surface expression.
      Phosphorylated predominantly on Ser residues.
    • Cellular localization
      Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
    • Information by UniProt
    • Database links
    • Alternative names
      • D11S4896E antibody
      • GOK antibody
      • OTTHUMP00000164512 antibody
      • OTTHUMP00000229140 antibody
      • OTTHUMP00000230742 antibody
      • SIM antibody
      • STIM 1 antibody
      • STIM1 antibody
      • Stim1 stromal interaction molecule 1 antibody
      • STIM1_HUMAN antibody
      • STIM1L antibody
      • Stromal interaction molecule 1 antibody
      see all


    • All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

      Lane 1 : SH SY5Y cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 77 kDa

    • ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.


    This product has been referenced in:
    • Yang S  et al. Store operated calcium channels are associated with diabetic cystopathy in streptozotocin-induced diabetic rats. Mol Med Rep 17:6612-6620 (2018). Read more (PubMed: 29532875) »
    • Wu G  et al. Overexpression of stromal interaction molecule 1 may promote epithelial-mesenchymal transition and indicate poor prognosis in gastric cancer. Mol Med Rep 16:151-158 (2017). IHC-P ; Human . Read more (PubMed: 28534934) »

    See all 5 Publications for this product

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