Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free (ab239938)

Overview

  • Product name

    Anti-Stromal interaction molecule 1 antibody [EPR3414] - BSA and Azide free
    See all Stromal interaction molecule 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3414] to Stromal interaction molecule 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue, cells or virus within Human Stromal interaction molecule 1 aa 650-750. The exact sequence is proprietary.

  • General notes

    Ab239938 is the carrier-free version of ab108994. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239938 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval with citrate buffer (pH 6): Heat up to 98°C, below boiling, and then let cool for 10-20 min.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 77 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
    • Tissue specificity

      Ubiquitously expressed in various human primary cells and tumor cell lines.
    • Involvement in disease

      Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
    • Sequence similarities

      Contains 1 EF-hand domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Domain

      The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
    • Post-translational
      modifications

      Glycosylation is required for cell surface expression.
      Phosphorylated predominantly on Ser residues.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.
    • Information by UniProt
    • Database links

    • Alternative names

      • D11S4896E antibody
      • GOK antibody
      • OTTHUMP00000164512 antibody
      • OTTHUMP00000229140 antibody
      • OTTHUMP00000230742 antibody
      • SIM antibody
      • STIM 1 antibody
      • STIM1 antibody
      • Stim1 stromal interaction molecule 1 antibody
      • STIM1_HUMAN antibody
      • STIM1L antibody
      • Stromal interaction molecule 1 antibody
      see all

    Images

    • All lanes : Anti-Stromal interaction molecule 1 antibody [EPR3414] (ab108994) at 1/1000 dilution

      Lanes 1 & 4 : Wild-type HAP1 whole cell lysate
      Lane 2 : STIM1 (Stromal interaction molecule 1) knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 77 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab108994 observed at 77 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab108994 was shown to specifically react with Stromal interaction molecule 1 in wild-type HAP1 cells as signal was lost in STIM1 (Stromal interaction molecule 1) knockout cells. Wild-type and STIM1 (Stromal interaction molecule 1) knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab108994 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108994).

    • ab108994 at 1/50 dilution, staining Stromal interaction molecule 1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108994).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab239938 has not yet been referenced specifically in any publications.

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