Strongyloides IgG/IgM ELISA kit (ab247203)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Overview
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Product name
Strongyloides IgG/IgM ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% sample 24 0.499nM 7.27% sample 24 1.21nM 4.42% sample 24 1.106nM 3.89% Inter-assay Sample n Mean SD CV% sample 12 31.77nM 10.72% sample 12 24.62nM 9.84% sample 12 8.68nM 9.69% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (qualitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Strongyloides IgG/IgM ELISA Kit (ab247203) is designed for the qualitative determination of IgG/IgM class antibodies against Strongyloides in human serum or plasma (citrate, heparin).
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent
Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
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Notes
Strongyloides is a genus containing some 50 species of obligate gastrointestinal parasites of vertebrates. Strongyloides stercoralis is the scientific name of a human parasitic roundworm causing the disease of strongyloidiasis. The Strongyloides stercoralis nematode can parasitize humans. The adult parasitic stage lives in tunnels in the mucosa of the small intestine. S. stercoralis can be found in areas with tropical and subtropical climates but cases also occur in temperate area, more frequently in rural areas. S. stercoralis has a very low prevalence in societies where fecal contamination of soil or water is rare. Many people infected are usually asymptomatic at first. Symptoms include dermatitis: swelling, itching, larva currens, and mild hemorrhage at the site where the skin has been penetrated. If the parasite reaches the lungs, the chest may feel as if it is burning, and wheezing and coughing may result, along with pneumonia-like symptoms (Löffler's syndrome). The intestines could eventually be invaded, leading to burning pain, tissue damage, sepsis, and ulcers. In severe cases, edema may result in obstruction of the intestinal tract, as well as loss of peristaltic contractions. Strongyloides infection in immunocompromised individuals (particularly following the administration of steroids, for example following transplant surgery) can result in disseminated strongyloidiasis, in which worms move beyond the confines of the gut into other organs.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml anti-human IgG/IgM HRP conjugate 1 x 20ml Cover Foil 1 unit Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strongyloides Coated Microplate 1 unit Strongyloides Cut-off Control: 1 x 3ml Strongyloides Negative Control 1 x 2ml Strongyloides Positive Control 1 x 2ml TMB Substrate Solution Yellow cap 1 x 15ml
Images
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab247203 has not yet been referenced specifically in any publications.