Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Zebrafish Tissue lysate - whole (whole zebrafish protein lysate)
Specification
whole zebrafish protein lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: ~140 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Feb 09 2015