Product nameAnti-Strumpellin antibody - C-terminal
See all Strumpellin primary antibodies
DescriptionRabbit polyclonal to Strumpellin - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Common marmoset, Orangutan, Elephant
- HeLa, 293T, Jurkat, mouse TCMK1, and mouse NIH 3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Abcam primary standard storage.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab206687 was affinity purified using an epitope specific to Strumpellin immobilized on solid support.
Our Abpromise guarantee covers the use of ab206687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 134 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionComponent of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting.
Tissue specificityExpressed ubiquitously.
Involvement in diseaseDefects in KIAA0196 are the cause of spastic paraplegia autosomal dominant type 8 (SPG8) [MIM:603563]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
Sequence similaritiesBelongs to the strumpellin family.
- Information by UniProt
- KIAA0196 antibody
- SPG8 antibody
- STRUM_HUMAN antibody
- WASH complex subunit strumpellin antibody
Detection of Strumpellin in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab206687 at 6 µg/mg lysate for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes : Anti-Strumpellin antibody - C-terminal (ab206687) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK1 whole cell lysate
Lane 5 : Mouse NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 134 kDa
Exposure time: 3 minutes
ab206687 has not yet been referenced specifically in any publications.