Overview

  • Product name

    Anti-Sts1 antibody [EPR16980]
    See all Sts1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16980] to Sts1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Sts1 aa 1-100. The exact sequence is proprietary.
    Database link: Q8TF42

  • Positive control

    • HepG2 and Jurkat whole cell lysate (ab7899); Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.
WB 1/2000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ICC/IF 1/280.

Target

  • Function

    Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.
  • Sequence similarities

    Contains 1 SH3 domain.
    Contains 1 UBA domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cbl interacting protein p70 antibody
    • Cbl interacting protein Sts 1 antibody
    • Cbl interacting protein Sts1 antibody
    • Cbl-interacting protein p70 antibody
    • KIAA1959 antibody
    • MGC15437 antibody
    • Nm23 phosphorylated unknown substrate antibody
    • p70 antibody
    • SH3 domain containing 70 kDa protein antibody
    • Sts 1 antibody
    • STS-1 antibody
    • STS1 antibody
    • Suppressor of T cell receptor signaling 1 antibody
    • Suppressor of T-cell receptor signaling 1 antibody
    • T-cell ubiquitin ligand 2 antibody
    • TULA-2 antibody
    • Tyrosine-protein phosphatase STS1/TULA2 antibody
    • Ubash3b antibody
    • Ubiquitin associated and SH3 domain containing B antibody
    • Ubiquitin-associated and SH3 domain-containing protein B antibody
    • UBS3B_HUMAN antibody
    see all

Images

  • All lanes : Anti-Sts1 antibody [EPR16980] (ab197027) at 1/2000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Sts1 with purified ab197027 at 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

     

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Sts1 with ab197027 at 1/280 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and weakly nuclear staining on Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab197027 at 1/280 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

References

This product has been referenced in:

  • Yang M  et al. K33-linked polyubiquitination of Zap70 by Nrdp1 controls CD8(+) T cell activation. Nat Immunol 16:1253-62 (2015). WB . Read more (PubMed: 26390156) »
See 1 Publication for this product

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