Recombinant Anti-Sts1 antibody [EPR16980] (ab197027)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Sts1 antibody [EPR16980]
See all Sts1 primary antibodies -
Description
Rabbit monoclonal [EPR16980] to Sts1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Sts1 aa 1-100. The exact sequence is proprietary.
Database link: Q8TF42 -
Positive control
- HepG2 and Jurkat whole cell lysate (ab7899); Jurkat cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16980 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab197027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50. | |
| WB | 1/2000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). | |
| ICC/IF | 1/280. |
Target
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Function
Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination. -
Sequence similarities
Contains 1 SH3 domain.
Contains 1 UBA domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84959 Human
- Omim: 609201 Human
- SwissProt: Q8TF42 Human
- Unigene: 444075 Human
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Alternative names
- Cbl interacting protein p70 antibody
- Cbl interacting protein Sts 1 antibody
- Cbl interacting protein Sts1 antibody
see all
Images
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![Western blot - Anti-Sts1 antibody [EPR16980] (ab197027)](https://www.abcam.com/ps/products/197/ab197027/Images/ab197027-236859-anti-sts1-antibody-epr16980-western-blot.jpg)
Western blot - Anti-Sts1 antibody [EPR16980] (ab197027)All lanes : Anti-Sts1 antibody [EPR16980] (ab197027) at 1/2000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry - Anti-Sts1 antibody [EPR16980] (ab197027)](https://www.abcam.com/ps/products/197/ab197027/Images/ab197027-262949-anti-sts1-antibody-epr16980-flow-cytometry.jpg)
Flow Cytometry - Anti-Sts1 antibody [EPR16980] (ab197027)Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Sts1 with purified ab197027 at 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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![Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (ab197027)](https://www.abcam.com/ps/products/197/ab197027/Images/ab197027-236860-anti-sts1-antibody-epr16980-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (ab197027)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Sts1 with ab197027 at 1/280 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and weakly nuclear staining on Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab197027 at 1/280 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Yang M et al. K33-linked polyubiquitination of Zap70 by Nrdp1 controls CD8(+) T cell activation. Nat Immunol 16:1253-62 (2015). WB . Read more (PubMed: 26390156) »
