RecombinantRabMAb

Recombinant Anti-Sts1 antibody [EPR16980] (ab197027)

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Western blot - Anti-Sts1 antibody [EPR16980] (ab197027)
  • Flow Cytometry - Anti-Sts1 antibody [EPR16980] (ab197027)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (ab197027)
  • Anti-Sts1 antibody [EPR16980] (ab197027)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16980] to Sts1
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Sts1 antibody [EPR16980]
    See all Sts1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16980] to Sts1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2 and Jurkat whole cell lysate (ab7899); Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab197027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/50.
WB
1/2000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ICC/IF
1/280.
Notes
Flow Cyt
1/50.
WB
1/2000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ICC/IF
1/280.

Target

  • Function

    Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.

  • Sequence similarities

    Contains 1 SH3 domain.
    Contains 1 UBA domain.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cbl interacting protein p70 antibody
      • Cbl interacting protein Sts 1 antibody
      • Cbl interacting protein Sts1 antibody
      • Cbl-interacting protein p70 antibody
      • KIAA1959 antibody
      • MGC15437 antibody
      • Nm23 phosphorylated unknown substrate antibody
      • p70 antibody
      • SH3 domain containing 70 kDa protein antibody
      • Sts 1 antibody
      • STS-1 antibody
      • STS1 antibody
      • Suppressor of T cell receptor signaling 1 antibody
      • Suppressor of T-cell receptor signaling 1 antibody
      • T-cell ubiquitin ligand 2 antibody
      • TULA-2 antibody
      • Tyrosine-protein phosphatase STS1/TULA2 antibody
      • Ubash3b antibody
      • Ubiquitin associated and SH3 domain containing B antibody
      • Ubiquitin-associated and SH3 domain-containing protein B antibody
      • UBS3B_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Sts1 antibody [EPR16980] (ab197027)
      Western blot - Anti-Sts1 antibody [EPR16980] (ab197027)
      All lanes : Anti-Sts1 antibody [EPR16980] (ab197027) at 1/2000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
      Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 73 kDa
      Observed band size: 73 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry - Anti-Sts1 antibody [EPR16980] (ab197027)
      Flow Cytometry - Anti-Sts1 antibody [EPR16980] (ab197027)

      Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Sts1 with purified ab197027 at 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (ab197027)
      Immunocytochemistry/ Immunofluorescence - Anti-Sts1 antibody [EPR16980] (ab197027)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Sts1 with ab197027 at 1/280 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and weakly nuclear staining on Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows;
      -ve control 1: ab197027 at 1/280 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Anti-Sts1 antibody [EPR16980] (ab197027)
      Anti-Sts1 antibody [EPR16980] (ab197027)

    References (2)

    Publishing research using ab197027? Please let us know so that we can cite the reference in this datasheet.

    ab197027 has been referenced in 2 publications.

    • Sang X  et al. Mutual regulations and breast cancer cell control by steroidogenic enzymes: Dual sex-hormone receptor modulation upon 17ß-HSD7 inhibition. J Steroid Biochem Mol Biol 193:105411 (2019). PubMed: 31207361
    • Yang M  et al. K33-linked polyubiquitination of Zap70 by Nrdp1 controls CD8(+) T cell activation. Nat Immunol 16:1253-62 (2015). WB . PubMed: 26390156

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