Key features and details
- Rabbit polyclonal to STUB1/CHIP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-STUB1/CHIP antibody
See all STUB1/CHIP primary antibodies
DescriptionRabbit polyclonal to STUB1/CHIP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Rhesus monkey, Bovine papillomavirus
Synthetic peptide within Human STUB1/CHIP (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q9UNE7
- WB: HEK-293T and HepG2 whole cell lysate. IHC-P: Mouse esophagus tissue; ES2 xenograft tissue. ICC/IF: HeLa cells.
Previously labelled as STUB1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 35 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionE3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
Tissue specificityHighly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 3 TPR repeats.
Contains 1 U-box domain.
DomainThe TPR domain is essential for ubiquitination mediated by UBE2D1.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
- Information by UniProt
- Antigen NY CO 7 antibody
- Antigen NY-CO-7 antibody
- C terminus of Hsp70-interacting protein antibody
All lanes : Anti-STUB1/CHIP antibody (ab228742) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
10% SDS-PAGE gel.
Paraffin-embedded mouse esophagus tissue stained for STUB1/CHIP using ab228742 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded ES2 xenograft tissue stained for STUB1/CHIP using ab228742 at 1/500 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for STUB1/CHIP (green) using ab228742 at 1/200 dilution in ICC/IF.
Nuclear counterstain: DNA probe (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228742 has not yet been referenced specifically in any publications.