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    stub1chip-antibody-ab229108.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-STUB1/CHIP antibody (ab229108)

  • Datasheet
  • SDS
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Western blot - Anti-STUB1/CHIP antibody (ab229108)
  • Western blot - Anti-STUB1/CHIP antibody (ab229108)
  • Western blot - Anti-STUB1/CHIP antibody (ab229108)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab229108)

Key features and details

  • Rabbit polyclonal to STUB1/CHIP
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human STUB1/CHIP protein (His tag) (ab206161)

View more associated products

Overview

  • Product name

    Anti-STUB1/CHIP antibody
    See all STUB1/CHIP primary antibodies
  • Description

    Rabbit polyclonal to STUB1/CHIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human STUB1/CHIP (internal sequence). The exact sequence is proprietary.
    Database link: Q9UNE7

  • Positive control

    • WB: HEK-293T whole cell lysate; Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts. IHC-P: Human breast cancer tissue.
  • General notes

    Previously labelled as STUB1.  

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E3 Ubiquitin Ligases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH 3T3 whole cell lysate (ab7179)
    • C2C12 whole cell lysate (ab7182)
    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant Human STUB1/CHIP protein (His tag) (ab206161)
  • Related Products

    • Recombinant Human STUB1/CHIP protein (ab139765)
    • Recombinant Human STUB1/CHIP protein (His tag) (ab206161)
    • Recombinant Human STUB1/CHIP protein (ab82791)

Applications

Our Abpromise guarantee covers the use of ab229108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 35 kDa.

Target

  • Function

    E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
  • Tissue specificity

    Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 3 TPR repeats.
    Contains 1 U-box domain.
  • Domain

    The TPR domain is essential for ubiquitination mediated by UBE2D1.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9UNE7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10273 Human
    • Entrez Gene: 56424 Mouse
    • Entrez Gene: 287155 Rat
    • Omim: 607207 Human
    • SwissProt: Q9UNE7 Human
    • SwissProt: Q9WUD1 Mouse
    • Unigene: 592081 Human
    • Unigene: 491120 Mouse
    • Unigene: 6504 Rat
    see all
  • Alternative names

    • Antigen NY CO 7 antibody
    • Antigen NY-CO-7 antibody
    • C terminus of Hsp70-interacting protein antibody
    • Carboxy terminus of Hsp70 interacting protein antibody
    • Carboxy terminus of Hsp70-interacting protein antibody
    • Carboxy terminus of Hsp70p interacting protein antibody
    • CHIP antibody
    • CHIP_HUMAN antibody
    • CLL associated antigen KW 8 antibody
    • CLL-associated antigen KW-8 antibody
    • E3 ubiquitin protein ligase CHIP antibody
    • E3 ubiquitin-protein ligase CHIP antibody
    • Heat shock protein A binding protein 2 (c terminal) antibody
    • HSPABP2 antibody
    • NY CO 7 antibody
    • PP1131 antibody
    • SDCCAG7 antibody
    • Serologically defined colon cancer antigen 7 antibody
    • STIP1 homology and U Box containing protein 1 antibody
    • STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
    • STIP1 homology and U box-containing protein 1 antibody
    • STUB 1 antibody
    • STUB1 antibody
    • UBOX 1 antibody
    • UBOX1 antibody
    see all

Images

  • Western blot - Anti-STUB1/CHIP antibody (ab229108)
    Western blot - Anti-STUB1/CHIP antibody (ab229108)
    Anti-STUB1/CHIP antibody (ab229108) at 1/10000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-STUB1/CHIP antibody (ab229108)
    Western blot - Anti-STUB1/CHIP antibody (ab229108)
    All lanes : Anti-STUB1/CHIP antibody (ab229108) at 1/10000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    12% SDS-PAGE gel.

  • Western blot - Anti-STUB1/CHIP antibody (ab229108)
    Western blot - Anti-STUB1/CHIP antibody (ab229108)
    All lanes : Anti-STUB1/CHIP antibody (ab229108) at 1/10000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    12% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab229108)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab229108)

    Paraffin-embedded human breast cancer tissue stained for STUB1/CHIP using ab229108 at 1/500 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab229108? Please let us know so that we can cite the reference in this datasheet.

    ab229108 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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