Anti-STUB1/CHIP antibody (ab229108)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-STUB1/CHIP antibody
See all STUB1/CHIP primary antibodies -
Description
Rabbit polyclonal to STUB1/CHIP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human STUB1/CHIP (internal sequence). The exact sequence is proprietary.
Database link: Q9UNE7 -
Positive control
- WB: HEK-293T whole cell lysate; Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts. IHC-P: Human breast cancer tissue.
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General notes
Previously labelled as STUB1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab229108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/5000 - 1/20000. Predicted molecular weight: 35 kDa. |
Target
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Function
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. -
Tissue specificity
Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 3 TPR repeats.
Contains 1 U-box domain. -
Domain
The TPR domain is essential for ubiquitination mediated by UBE2D1. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10273 Human
- Entrez Gene: 56424 Mouse
- Entrez Gene: 287155 Rat
- Omim: 607207 Human
- SwissProt: Q9UNE7 Human
- SwissProt: Q9WUD1 Mouse
- Unigene: 592081 Human
- Unigene: 491120 Mouse
see all -
Alternative names
- Antigen NY CO 7 antibody
- Antigen NY-CO-7 antibody
- C terminus of Hsp70-interacting protein antibody
see all
Images
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Western blot - Anti-STUB1/CHIP antibody (ab229108)Anti-STUB1/CHIP antibody (ab229108) at 1/10000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 35 kDa10% SDS-PAGE gel.
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Western blot - Anti-STUB1/CHIP antibody (ab229108)All lanes : Anti-STUB1/CHIP antibody (ab229108) at 1/10000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa12% SDS-PAGE gel.
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Western blot - Anti-STUB1/CHIP antibody (ab229108)All lanes : Anti-STUB1/CHIP antibody (ab229108) at 1/10000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa12% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab229108)Paraffin-embedded human breast cancer tissue stained for STUB1/CHIP using ab229108 at 1/500 dilution in immunohistochemical analysis.
References
ab229108 has not yet been referenced specifically in any publications.
