Recombinant
RabMAb

Recombinant Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)

Overview

  • Product name

    Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free
    See all STUB1/CHIP primary antibodies
  • Description

    Rabbit monoclonal [EPR4447] to STUB1/CHIP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human STUB1/CHIP aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human skeletal muscle tissue.
  • General notes

    ab238966 is the carrier-free version of ab134064 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab238966 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as STUB1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
  • Tissue specificity

    Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 3 TPR repeats.
    Contains 1 U-box domain.
  • Domain

    The TPR domain is essential for ubiquitination mediated by UBE2D1.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen NY CO 7 antibody
    • Antigen NY-CO-7 antibody
    • C terminus of Hsp70-interacting protein antibody
    • Carboxy terminus of Hsp70 interacting protein antibody
    • Carboxy terminus of Hsp70-interacting protein antibody
    • Carboxy terminus of Hsp70p interacting protein antibody
    • CHIP antibody
    • CHIP_HUMAN antibody
    • CLL associated antigen KW 8 antibody
    • CLL-associated antigen KW-8 antibody
    • E3 ubiquitin protein ligase CHIP antibody
    • E3 ubiquitin-protein ligase CHIP antibody
    • Heat shock protein A binding protein 2 (c terminal) antibody
    • HSPABP2 antibody
    • NY CO 7 antibody
    • PP1131 antibody
    • SDCCAG7 antibody
    • Serologically defined colon cancer antigen 7 antibody
    • STIP1 homology and U Box containing protein 1 antibody
    • STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
    • STIP1 homology and U box-containing protein 1 antibody
    • STUB 1 antibody
    • STUB1 antibody
    • UBOX 1 antibody
    • UBOX1 antibody
    see all

Images

  • Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling STUB1/CHIP with purified ab134064 at 1/120 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134064).

  • ab134064 (purified) at 1/120 immunoprecipitating STUB1/CHIP in 10 μg HeLa (Lanes 1 and 2, observed at 35 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134064).

  • Immunofluorescence staining of SH-SY5Y cells with purified ab134064 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab134064 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134064).

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labelling STUB1/CHIP with unpurified ab134064 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab134064).

References

ab238966 has not yet been referenced specifically in any publications.

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