Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
1000 µg
Immuno-precipitation step
Protein A/G
Specification
HeLa
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% Triton in TBS
Dilution
1/200
Western Blot antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Millipore 100000
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Additional data
Additional Notes
4447 CHIP = IP performed using ab134064 (Stub1 EPR 4447)
4448 CHIP = IP performed using ab109103 (Stub1 EPR 4448)
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Submitted Apr 11 2013