Anti-STX11 antibody (ab69828)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-STX11 antibody
See all STX11 primary antibodies -
Description
Mouse polyclonal to STX11 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
STX11 (NP_003755.2, 1 a.a. ~ 287 a.a) full-length human protein.
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Positive control
- Human spleen and STX11 transfected 293T cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab69828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. -
Involvement in disease
Defects in STX11 are the cause of hemophagocytic lymphohistiocytosis familial type 4 (FHL4) [MIM:603552]; also known as HPLH4. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network membrane. - Information by UniProt
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Database links
- Entrez Gene: 8676 Human
- Omim: 605014 Human
- SwissProt: O75558 Human
- Unigene: 118958 Human
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Alternative names
- FHL4 antibody
- HLH4 antibody
- HPLH4 antibody
see all
Images
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Western blot - Anti-STX11 antibody (ab69828)Anti-STX11 antibody (ab69828) at 1/500 dilution + human spleen lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 33 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-STX11 antibody (ab69828)All lanes : Anti-STX11 antibody (ab69828) at 1/500 dilution
Lane 1 : STX11 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Hellewell AL et al. Analysis of familial hemophagocytic lymphohistiocytosis type 4 (FHL-4) mutant proteins reveals that S-acylation is required for the function of syntaxin 11 in natural killer cells. PLoS One 9:e98900 (2014). Human . Read more (PubMed: 24910990) »
- Izbicka E et al. Plasma biomarkers distinguish non-small cell lung cancer from asthma and differ in men and women. Cancer Genomics Proteomics 9:27-35 (2012). WB ; Human . Read more (PubMed: 22210046) »
