Anti-STX17 antibody (ab229646)
Key features and details
- Rabbit polyclonal to STX17
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-STX17 antibody
See all STX17 primary antibodies -
Description
Rabbit polyclonal to STX17 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human STX17 (internal sequence). The exact sequence is proprietary.
Database link: P56962 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Implicated in vesicle trafficking to lysosomes. Could be involved in processes related to cell division. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Membrane. Appears to be associated with a membrane compartment, perhaps a subset of the ER such as exit or entrance sites. - Information by UniProt
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Database links
- Entrez Gene: 55014 Human
- Entrez Gene: 67727 Mouse
- Entrez Gene: 252853 Rat
- Omim: 604204 Human
- SwissProt: P56962 Human
- SwissProt: Q9D0I4 Mouse
- SwissProt: Q9Z158 Rat
- Unigene: 704031 Human
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Alternative names
- FLJ20651 antibody
- MGC102796 antibody
- MGC126613 antibody
see all
Images
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All lanes : Anti-STX17 antibody (ab229646) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa12% SDS-PAGE
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Paraffin-embedded mouse prostate tissue staining STX17 using ab229646 at 1/500 dilution in immunohistochemical analysis.
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Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling STX17 (green) with ab229646 at 1/500 dilution. Blue: Fluoroshield with DAPI. Scale bar= 10 μm.
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All lanes : Anti-STX17 antibody (ab229646) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa12% SDS-PAGE
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Immunofluorescence analysis of 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons labeling STX17 (green) with ab229646 at 1/500 dilution. Red: beta Tubulin 3, using beta Tubulin 3 antibody at 1/500 dilution. Blue: DAPI.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab229646 has been referenced in 5 publications.
- Zhu Y et al. Targeting Aβ and p-Tau Clearance in Methamphetamine-Induced Alzheimer's Disease-Like Pathology: Roles of Syntaxin 17 in Autophagic Degradation in Primary Hippocampal Neurons. Oxid Med Cell Longev 2022:3344569 (2022). PubMed: 35633882
- Sano M et al. Neutrophil extracellular traps-mediated Beclin-1 suppression aggravates atherosclerosis by inhibiting macrophage autophagy. Front Cell Dev Biol 10:876147 (2022). PubMed: 35923856
- Dewi FRP et al. Nucleoporin TPR (translocated promoter region, nuclear basket protein) upregulation alters MTOR-HSF1 trails and suppresses autophagy induction in ependymoma. Autophagy 17:1001-1012 (2021). PubMed: 32207633
- An Z & Ding W Acinetobacter baumannii up-regulates LncRNA-GAS5 and promotes the degradation of STX17 by blocking the activation of YY1. Virulence 12:1965-1979 (2021). PubMed: 34304694
- Shen S et al. BRD4 Inhibition Protects Against Acute Pancreatitis Through Restoring Impaired Autophagic Flux. Front Pharmacol 11:618 (2020). PubMed: 32457617