- Datasheet
- Specific References (16)
- Protocols
Overview
-
Product nameAnti-Substance P antibody
See all Substance P primary antibodies -
DescriptionGuinea pig polyclonal to Substance P
-
Host speciesGuinea pig
-
Tested applicationsSuitable for: IHC-FoFr, ICC/IF, IHC-Pmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide:
CRPKPQQFFGLM
, corresponding to amino acids 1-11 of Rat Substance P (Peptide available as ab38217.) -
General notes
This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Alternative splicing of exons 4 and/or 6 produces four known products of undetermined significance.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: Whole Serum -
Concentration information loading... -
PurityImmunogen affinity purified
-
Primary antibody notesThis gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Alternative splicing of exons 4 and/or 6 produces four known products of undetermined significance.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab10353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | 1/1000. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
-
FunctionTachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
-
Sequence similaritiesBelongs to the tachykinin family.
-
Cellular localizationSecreted.
- Information by UniProt
-
Database links
- Entrez Gene: 6863 Human
- Entrez Gene: 21333 Mouse
- Entrez Gene: 24806 Rat
- Omim: 162320 Human
- SwissProt: P20366 Human
- SwissProt: P41539 Mouse
- SwissProt: P06767 Rat
- Unigene: 2563 Human
see all -
Alternative names
- C-terminal-flanking peptide antibody
- Hs.2563 antibody
- neurokinin 1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody (ab10353)This image is courtesy of an Abreview submitted by Carl Hobbsab10353 staining Substance P in Human brain stem tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 2 hours at 22°C. A Biotin-conjugated Rabbit anti-guinea pig polyclonal (1/200) was used as the secondary antibody.
-
Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody (ab10353)Immuofluorescent detection of Substance P in rat spinal cord dorsal horn (red fluorescence). DAPI (blue) was used as counter stain. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Substance P antibody (ab10353)Image from Jeffry JA et al., PLoS One. 2009 Sep 15;4(9):e7021. doi: 10.1371/journal.pone.0007021. Fig 6.; September 15, 2009, PLoS ONE 4(9): e7021.Immunohistochemical analysis of rat paw skin tissue, staining Substance P with ab10353 at 1/1000 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody (ab10353)This image is courtesy of an Abreview submitted by Carl Hobbsab10353 staining Substance P in Mouse brain stem tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/400 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Rabbit anti-Guinea pig polyclonal (1/200) was used as the secondary antibody.
-
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Substance P antibody (ab10353)ab10353 staining Substance P in mouse spinal cord (red fluorescence) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue was counterstained with Fluoro Nissl Green (green fluorescence). -
Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody (ab10353)Sustance P staining of trigeminal ganglia from P3-P5 rat pups.
References
This product has been referenced in:
- Nagy JI et al. Connexin36 Expression in Primary Afferent Neurons in Relation to the Axon Reflex and Modality Coding of Somatic Sensation. Neuroscience 383:216-234 (2018). Read more (PubMed: 29746988) »
- Wan L et al. Distinct roles of NMB and GRP in itch transmission. Sci Rep 7:15466 (2017). Read more (PubMed: 29133874) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"