Anti-Substance P antibody (ab10353)
Key features and details
- Guinea pig polyclonal to Substance P
- Suitable for: IHC-FoFr, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Substance P antibody
See all Substance P primary antibodies -
Description
Guinea pig polyclonal to Substance P -
Host species
Guinea pig -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-FoFr Mouse
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Immunogen
Synthetic peptide:
CRPKPQQFFGLM
, corresponding to amino acids 1-11 of Rat Substance P (Peptide available as ab38217.) -
General notes
This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Alternative splicing of exons 4 and/or 6 produces four known products of undetermined significance.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Primary antibody notes
This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Alternative splicing of exons 4 and/or 6 produces four known products of undetermined significance. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Immunizing Peptide (Blocking)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Rat
|
| IHC-FoFr |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr |
1/1000.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-FoFr
1/1000. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. -
Sequence similarities
Belongs to the tachykinin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 21333 Mouse
- Entrez Gene: 24806 Rat
- SwissProt: P41539 Mouse
- SwissProt: P06767 Rat
- Unigene: 1440 Mouse
- Unigene: 410823 Mouse
- Unigene: 1920 Rat
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Alternative names
- C-terminal-flanking peptide antibody
- Hs.2563 antibody
- neurokinin 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody (ab10353)Immuofluorescent detection of Substance P in rat spinal cord dorsal horn (red fluorescence). DAPI (blue) was used as counter stain.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Substance P antibody (ab10353)Image from Jeffry JA et al., PLoS One. 2009 Sep 15;4(9):e7021. doi: 10.1371/journal.pone.0007021. Fig 6.; September 15, 2009, PLoS ONE 4(9): e7021.Immunohistochemical analysis of rat paw skin tissue, staining Substance P with ab10353 at 1/1000 dilution. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Substance P antibody (ab10353)ab10353 staining Substance P in mouse spinal cord (red fluorescence) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue was counterstained with Fluoro Nissl Green (green fluorescence). -
Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody (ab10353)Sustance P staining of trigeminal ganglia from P3-P5 rat pups.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab10353 has been referenced in 27 publications.
- Barry DM et al. Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors. Nat Commun 11:1397 (2020). PubMed: 32170060
- Khom S et al. Alcohol dependence potentiates substance P/neurokinin-1 receptor signaling in the rat central nucleus of amygdala. Sci Adv 6:eaaz1050 (2020). PubMed: 32206720
- Dolan CP et al. Axonal regrowth is impaired during digit tip regeneration in mice. Dev Biol 445:237-244 (2019). PubMed: 30458171
- Richter F et al. Optogenetic augmentation of the hypercholinergic endophenotype in DYT1 knock-in mice induced erratic hyperactive movements but not dystonia. EBioMedicine 41:649-658 (2019). PubMed: 30819512
- Magadmi R et al. Secretion of Mast Cell Inflammatory Mediators Is Enhanced by CADM1-Dependent Adhesion to Sensory Neurons. Front Cell Neurosci 13:262 (2019). PubMed: 31275114